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Distinct heat-shock element arrangements that mediate the heat
Distinct heat-shock element arrangements that mediate the heat
shock, but not the late-embryogenesis induction of small
heat-shock proteins, correlate with promoter activation
in root-knot nematode feeding cells
Marta Barcala ? Alejandra Garc??a ? Pilar Cubas ? Concepcio?n Almoguera ?
Juan Jordano ? Carmen Fenoll ? Carolina Escobar
Received: 19 July 2007 / Accepted: 2 November 2007 / Published online: 28 November 2007
Springer Science+Business Media B.V. 2007
Abstract Genes coding small heat-shock proteins
(sHSPs) show distinct behaviours with respect to environ-
mental and developmental signals. Their transcriptional
regulation depends on particular combinations of heat
stress cis-elements (heat-shock elements; HSEs) but many
aspects regarding their regulation remain unclear. Cyst and
root-knot nematodes induce, in the roots of infected plants,
the differentiation of special feeding cells with high met-
abolic activity (syncytia and giant cells, respectively), a
process accompanied by extensive gene expression chan-
ges. The Hahsp17.7G4 (G4) promoter was active in giant
cells and its HSE arrangements were crucial for this acti-
vation. In the present work, we provide further basis to
associate giant cell expression with the heat-shock
response of this gene class, by analysing additional pro-
moters. The Hahsp17.6G1 (G1) promoter, not induced by
heat shock, was silent in giant cells, while Hahsp18.6G2
(G2), which responds to heat shock, was specifically
induced in giant cells. In addition, a mutated Hahsp17.7G4
promoter version (G4MutP) with a strong heat-shock
induction was also induced in giant cells. The responses of
the different promoters correlated with distinct HSE con-
figurations, which might have implications on differential
trans-activation. Furthermore, the shortest giant cell and
heat-shock-inducible sHSP promoter version analysed in
tobacco (-83pb Hahsp17.7G4) fully maintained its
expression profile in Arabidopsis. Cyst nematodes did not
induce the H
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