p3xFLAG-CMV-10哺乳动物表达载体说明.pdfVIP

p3xFLAG-CMV-10 编号 ? 载体名称 ? 北京华越洋生物 VECT6030 ? p3xFLAG--‐CMV--‐10 ? p3xFLAG--‐CMV--‐10载体基本信息 载体名称 : p3XFLAG-CMV-10 质粒类型 : 哺乳动物表达载体 高拷贝 /低拷贝 : 低拷贝 克隆方法 : 多克隆位点；限制性内切酶 启动子 : CMV 载体大小 : 6299 bp 5 测序引物及序列 : CMV Forward：CGCAAATGGGCGGTAGGCGTG 3 测序引物及序列 : -- 载体标签 : 3XFLAG tag (N-末端） 载体抗性 : 氨苄青霉素 筛选标记 : Neomycin 克隆菌株 : -- 宿主细胞（系） : 常用细胞系，如293、Hela等 备注 : CMV启动子驱动目的蛋白高水平表达； FLAG标签含8个氨基酸残基（DYKDDDDK），3XFLAG标签含22个氨基酸 残基，一般位于融合蛋白的外表面，具有高度免疫灵敏性，便于检测和纯化目的蛋白； 用EK蛋白酶可将3XFLAG标签切除。 稳定性 : 瞬表达或稳表达 组成型 /诱导型 : 组成型 病毒 /非病毒 : 非病毒 p3xFLAG--‐CMV--‐10载体质粒图谱和多克隆位点信息 p3xFLAG--‐CMV--‐10载体简介 FLAG 和 3xFLAG 标签： A Proven System for Detection and Purification of Proteins The FLAG Expression System is an established way to express, purify and detect recombinant fusion proteins. FLAG and 3xFLAG have proven utility in numerous applications such as Western blotting, immunocytochemistry, immunoprecipitation, flow cytometry, protein purification, and in the study of protein-protein interactions, cell ultrastructure and protein localization. These small hydrophilic tags facilitate superior detection and purification of recombinant fusion proteins when using our highly specific and sensitive ANTI-FLAG antibodies. Ideal Epitope Tags: Small, Hydrophilic and Cleavable The FLAG expression system utilizes a short, hydrophilic 8-amino acid peptide that is fused to the recombinant protein of interest. Because of its hydrophilic nature, the FLAG peptide is likely to be located on the surface of the fusion protein. As a result, the FLAG peptide is easily accessible for cleavage by enterokinase (Ek) and for detection with antibodies. In addition, because of the small size of the FLAG peptide tag, it is not likely to obscure other epitopes, domains, or alter function, secretion, or transport of the fusion protein. The 3xFLAG system is an improvement upon the original system by fusing 3 tandem FLAG epitopes (22 amino acids). Detection of fusion proteins containin