CXCR7在SDF-1介导内皮细胞参与血管生成中作用-第三军医大学学报.doc

CXCR7在SDF-1介导内皮细胞参与血管生成中作用研究 熊新1，李红2，潘克俭2，王兰2，李亚2，戴小珍2基金项目：国家自然科学基金 ? 通讯作者：戴小珍，E-mail：xiaozhendai@ (1. 重庆医科大学第一附属医院实验研究中心，重庆市 400016; 2. 成都医学院生物医学系，成都市 610500) 【摘要】 目的：探讨CXCR7在SDF-1诱导内皮细胞参与血管生成中的作用，以期为血管相关疾病的治疗提供新的治疗靶点。方法：用western-blot免疫印迹法和流式细胞术法检测脐静脉内皮细胞（HUVECs）中CXCR7和CXCR4的表达情况；利用小分子拮抗剂分别阻断CXCR4或CXCR7，然后通过MTT法、transwell小室法及三维基质胶血管样结构形成实验分别考察CXCR7和CXCR4在SDF-1诱导HUVECs 增殖、迁移及管样结构形成能力的影响。结果：HUVECs 细胞中同时高表达CXCR4和CXCR7这两个受体；SDF-1在调节HUVECs的增殖是通过与CXCR7相互作用而实现的，但CXCR7不参与SDF-1诱导HUVECs的迁移过程；在SDF-1诱导HUVECs形成血管样结构的过程中，CXCR7和CXCR4均发挥着重要作用，缺一不可。结论：CXCR7和CXCR4在SDF-1诱导HUVECs参与血管生成的过程中均起着重要的作用，但这两者的作用不尽一致，这为以前关于SDF-1诱导血管生成的作用机制提供新的认识，同时以期为血管相关疾病的治疗提供新作用靶点，但CXCR7的作用机理还需进一步的研究和证明。 关键词：CXCR7；基质衍生衍生因子（SDF-1）；内皮细胞；血管生成 中图分类号：Q26；Q291 The role of CXCR7 in angiogenesis of endothelial cells induced by SDF-1 Xiong Xin1, Li Hong2, Pan Kejian2, Wang Lan2, Dai Xiaozhen2* (1. Laboratory Research Center, The First Affiliated Hospital, Chongqing Medical University; Chongqing 400016, China; 2. The school of biomedicine, Chengdu Medical College, Chengdu, 610500, China ) [Abstract] Objective：To explore the role of CXCR7 in the angiogenesis of endothelial cells induced by SDF-1 and hope to find a new target molecular for angiogenesis-dependent diseases. Methods: The expression of CXCR7 and CXCR4 in HUVECs were examined by western blot assay and flow cytometry assay. The effects of CXCR4 and CXCR7 on HUVECs’ proliferation, migration and forming tube-like structures induced by SDF-1 were tested by MTT, transwell assay, 3D-gel tube formation assay respectively. Result: Both CXCR4 and CXCR7were expressed highly in HUVECs. The proliferation of HUVECs induced by SDF-1 was inhibited by blocking either CXCR4 or CXCR7 with antagonists. SDF-1 regulated the migration of EPCs via CXCR4 but not CXCR7. However, Both CXCR7 and CXCR4 are essential for the tube formation of HUVECs induced by SDF-1. Conclusion: These results suggested that CXCR7 plays an important role in angiogenesis of HUVECs induced