食品安全研究进展课件1.pptx

Antimicrobial Activity of Xoconostle Pears (Opuntia matudae)against Escherichia coli O157:H7 in Laboratory Medium;;;目录;;大肠埃希菌 Escherichia coli O157:H7 ; Escherichia coli O157:H7 ;Foodborne pathogens (食源性致病菌)are major concern to consumers, food, industry, and food regulatory agencies. The yearly cost of foodborne illnesses in the United States as reported in 2010 was about $152 billion including $993 million caused by Escherichia coli O157:H7 in healthcare. E. coli O157:H7 is one of the most important foodborne pathogens in the United States, having been first identified in 1982. From 1982 to 2002, E. coli O157:H7 caused 73,000 illnesses annually in the United States including 8,598 infection cases and 40 deaths.;Therefore, the control and prevention of E. coli O157:H7 in food products is an area that is receiving worldwide attention. Chemical preservatives have been used for years, later considered to be unhealthy. Therefore, natural antimicrobials demand has been increased. Natural antimicrobials can be found in a variety of plants including herbs, spices, fruits, vegetables, and tropical plants. Food industries are very motivated to replace chemical preservatives with natural antimicrobials.;Xoconostle pears’s (Opuntia matudae) strong anticancer, antidiabetic, and antioxidant characteristics have attracted the attention of researchers around the world. rich source of soluble phenolics, ascorbic acid, and betalains effective antimicrobial activity Thus xoconostle pears have great potential as natural antimicrobial. The objective of this study was to examine the antimicrobial activity of xoconostle pears direct extract against E. coli O157:H7 in brain heart infusion (BHI) laboratory medium.;;a.实验细菌制备Bacterial Culture Activation and Preparation;是一种将高分辨率凝胶电泳和免疫化学分析技术相结合的杂交技术。 免疫印迹法分三个阶段 SDS-聚丙烯酰胺凝胶电泳； 电转移—将在凝胶 中已经分离的条带转移至硝酸纤维素膜上，选用低电压（100V）和大电流（1～2A），通电45min ； 酶免疫定位—将印有蛋白质条带的硝酸纤维素膜（相当于包被了抗原的固相载体）依次与特异性抗体和酶标第二抗体作用后，加入能形成不溶性显色物的酶反应底物，使区带染色。;a.实验细菌制备Bacterial Cultur