Acanthopanax senticosus glycosides in vitro production of B E of.docVIP

 PAGE \* MERGEFORMAT 12 Acanthopanax senticosus glycosides in vitro production of B E of Of: Xingzhao Bin, Wang Mingyan, Wu Peng, Feng Yun Lo [Abstract] Objective To investigate 2,4-D and the medium on Callus and somatic embryo production senticosus glycosides B, E effect, to create an enabling senticosus glycosides B, E accumulation of Acanthopanax in vitro culture system. Methods from the same explants Callus and somatic embryos were induced proliferation or produce secondary somatic embryos, HPLC determination of Acanthopanax glycosides in culture B, E content and quality of culture investigated type of culture, media types and hormones on Acanthopanax Gan B, E production. the result of somatic embryo quality is not lower than the quality of callus, but somatic embryo production Acanthopanax Gan B, the ability of E callus .2,4-D than on senticosus glycosides B, E content of low, only changed the culture of quality. MS treatment in the culture medium glycoside of Acanthopanax B, E yield better than 1/2MS medium. Conclusions In vitro cultures of Acanthopanax senticosus glycosides can produce B, E, its production depends on the type of culture, media types and 2,4-D concentration. [Keywords:] senticosus, Acanthopanax glycoside B, senticosus glycosides E, callus, somatic embryos Abstract: ObjectiveTo discuss the effect of 2,4-D and culture medium to the callus and somatic embryos for the production of eleutherosides B, E, establish in vitro raise system of Eleutherococcus senticosus Harms.MethodsUsing Callus and somatic embryos which came from the same explant to induce multiplication or to produce secondary somatic embryos. Determined the content of eleutherosides B, E and the weight of culture medium by HPLC. The effect of eleutherosides B, E by the cultures type, the culture medium kind and the hormone were studied. ResultsEven though the weight of somatic embryos was slightly lower than that of the callus, the ability of somatic embryos to produc