After cutting Qiong Long Haima umbrella differences in rat hippocampus in 83 ku protein mass spectrometry.docVIP

 PAGE \* MERGEFORMAT 15 After cutting Qiong Long Haima umbrella differences in rat hippocampus in 83 ku protein mass spectrometry Of: Zhuhui Xia, Qin Jianbing, Jin Guohua, Tian Meiling, Zhang Xinhua, Huang Zhen [Abstract] Objective: To investigate the cut dome umbrella Long Haima hippocampus neural stem cells to induce neuronal differentiation between biological activity of 83 ku protein composition and function. Methods: SD rats were cut 14 d after the umbrella dome Long Haima hippocampal non-denaturing polyacrylamide gel electrophoresis (Native PAGE), application of electrospray ionization mass spectrometry, protein database and literature analysis, differences of 83 ku protein was detected and functional analysis. Results: MS analysis of peptides found 17 group, according to these proteins its function can be divided into: cytoskeletal protein, enzyme in metabolism, signal transduction, protein degradation, oxidative stress, neurotransmitter transport, synapse formation and function of unknown proteins. Conclusion: Long Haima umbrella dome cutting between 83 ku protein in the hippocampus can Rho signaling pathway through participation, regulation of neural stem cells differentiate into neurons. [Keywords:] 83 ku protein, cutting Qiong Long Haima umbrella, hippocampus, non-denaturing polyacrylamide gel electrophoresis, mass spectrometry [Abstract] Objective: To explore the ingredients and functions of the active 83 ku proteins inducing NSCs to differentiate into neurons from the fimbria / fornix transected hippocampus.Methods: The Sprague Dawley rats’ hippocampi on the 14th day after fimbria / fornix transection were used for Native PAGE. Electrospray ionization mass spectrometry, protein data bank and bibliometrics were applied to identify the 83 ku proteins and analyze their functions. Results: Mass spectrometry analysis of 83 ku protein found 17 groups of peptides which were divided into several functional groups: cytoskeletal protein, enzyme i