Ching Ling consolidate Astragaloside film solid phase extraction - high performance liquid chromatography - evaporative light scattering determination of.docVIP

 PAGE \* MERGEFORMAT 10 Ching Ling consolidate Astragaloside film solid phase extraction - high performance liquid chromatography - evaporative light scattering determination of Of: Douyu Hong, Cui Jian force, Huang Yun, Li Qing [Abstract] Objective To consolidate the film Ching Ling determination astragaloside. Methods Solid phase extraction - high performance liquid chromatography - evaporative light scattering detection (SPE-HPLC-ELSD), Agilent-C18 column (4.6 mm 250 mm, 5 m), mobile phase methanol - water (74:26), column temperature 35 , the flow rate 0.8 ml min-1. Results astragaloside range of 0.272 ~ 5.432 g good linear relationship , r = 0.999 9, the average recovery was 98.82%, RSD was 1.57% (n = 9. Conclusion The method is simple, rapid, sensitive, accurate, reproducible and can be used as Ching Ling quality control of solid film . [Keywords:] Chen Ling solid film, solid phase extraction, HPLC, astragaloside Abstract: ObjectiveTo build up a method for determination of astragaloside in Zhenlingguben tablets. MethodsSPE-HPLC-ELSD was used in the quantitative analysis with Agilent C18 chromatography column (4.6 mm 250 mm, 5m) and methanol-water (74:26) as a mobile phase. The flow rate of mobile phase was 0.8 ml min-1. The column temperature was set to 35 . ResultsThe linear range for astragaloside was 0.272 ~ 5.432g (r = 0.999 9), the average recovery was 98.82% (RSD = 1.57%, n = 9). ConclusionThis method is simple, rapid, reproducible, and can be regarded as the quality control method of Zhenlingguben tablets. Keywords:: Zhenlingguben Tablets; Solid-phase extraction; HPLC; Astragaloside Ching Ling solid film that I corrected in the development of new drugs reported, mainly by Ligustrum lucidum, Ganoderma lucidum, astragalus and other traditional Chinese medicine processing, with Qi detoxification, Sanjie swelling, soothe the nerves and stomach effectiveness of chemotherapy for cancer cause of leukopenia, thrombocytopenia, immune