Chronic obstructive pulmonary disease in the expression of AQP4 mRNA and with studies of airway inflammation.docVIP

 PAGE \* MERGEFORMAT 15 Chronic obstructive pulmonary disease in the expression of AQP4 mRNA and with studies of airway inflammation [Abstract] Objective To investigate the water channel 4 (apuaporin 4, AQP4) mRNA in patients with chronic obstructive pulmonary disease (COPD) expression and chronic airway inflammation on the impact and significance. Methods for various reasons, was a pathologist bronchoscopy confirmed to be normal bronchial mucosa persons and each of chronic obstructive pulmonary disease patients, 30 cases were normal and COPD groups. testing lung function, bronchoscopy in parallel, the Qu mucosa by reverse transcription PCR, mRNA expression, HE staining of bronchial mucosal inflammatory cell infiltration. Results Compared with normal control group, COPD patients AQP4 mRNA expression in bronchial mucosa decreased (P lt;0.05), and negatively correlated with the degree and the inflammation (r =- 0.493, P lt;0.01), and FEV1/FVC , FEV1/pred% positive correlation (r = 0.405,0.461, P all lt;0.01). aquaporin mRNA expression in patients with COPD reduced the impact of airway humidification function, increased airway inflammation, to promote the development of COPD. [Keywords:] Chronic obstructive pulmonary disease, water channel, airway inflammation ABSTRACT: Objective To explore the effect and significance of AQP4 mRNA expression in bronchial epithelium of chronic obstructive pulmonary disease (COPD) on airway inflammation. Methods Bronchial epithelium was obtained by bronchoscopy in patients with COPD (n = 30) and healthy controls (n = 30) whose bronchial epithelium was confirmed to be normal by the pathologist. AQP4 mRNA were studied by RT PCR method. Inflammatory cells infiltration was observed by HE staining; pulmonary function test was performed too before bronchoscopy. Results Compared with that in the healthy controls, AQP4 mRNA expression in COPD was reduced (P lt;0.05). Furthermore, AQP4 mRNA expression was correlated negatively with