Electric with cerebral hypoxia-ischemia in neonatal rat hippocampus the expression of Nestin and Brdu.docVIP

 PAGE \* MERGEFORMAT 9 Electric with cerebral hypoxia-ischemia in neonatal rat hippocampus the expression of Nestin and Brdu [Abstract] Objective: To observe the electroacupuncture on cerebral hypoxic-ischemic neonatal rat hippocampus in neuroepithelial stem cell protein (Nestin) and 5 - bromo -2 - deoxy-uracil (Brdu) expression, understanding of power against cerebral anoxia neurogenesis after ischemia the level of intervention, for the electro-acupuncture therapy points to provide experimental basis for clinical application. Methods: 100 healthy seven-day-old Wistar rats weighing 12 ~ 15 g, were randomly divided into four groups: control group, model to determine group, hypoxic-ischemic group and the hypoxic-ischemic electro-acupuncture group, each group animals (except the blank control group outside) were the left common carotid artery ligation, and then the animals placed in 8% O2-92% N2 gas mixture in 2 h after the removal. Model to determine the hypoxic-ischemic animals were killed after 48 h to prepare brain tissue. Cerebral hypoxia-ischemia electroacupuncture group in the hypoxic-ischemic recovery 2 d, began to stimulate the ‘Baihui’ and ‘Ojo’ two points. In addition to model determined 24 d after the group, each group of animals were produced in brain tissue samples taken on the left, line Nestin and Brdu immunohistochemical staining. Results: Cerebral hypoxic-ischemic hippocampal CA1 area electro-acupuncture group Nestin immunoreactive cells is higher than the normal control group and hypoxic-ischemic group (P amp;lt;0.05). Cerebral hypoxic-ischemic dentate gyrus of the electroacupuncture group Brdu immunoreactive cells is higher than the normal control group and higher than the hypoxic-ischemic group (P amp;lt;0.05). Conclusion: The electro-acupuncture acupuncture treatment can significantly increase (strengthening) after cerebral hypoxic-ischemic hippocampal neurogenesis levels (capacity). [Keywords:] Electroacupuncture; hypoxia-ischemia; Nestin;