Electrospray ionization mass spectrometry analysis of cyclosporin structure of multi-stage.docVIP

 PAGE \* MERGEFORMAT 14 Electrospray ionization mass spectrometry analysis of cyclosporin structure of multi-stage Author: Chen Zhenwei Wenyao Ming Ji Xin-Yan Zheng Abstract Application of electrospray ionization multistage mass spectrometry analysis of four kinds of cyclosporin congeners, and the laws of their decomposition analysis of induction. In the electrospray ionization multi-stage mass spectrometry under the conditions of the formation of N terminal plus cyclosporine first proton [MH] molecular ion, and then along the peptide chain amide bond rupture, resulting in a corresponding fragment ions, the open-loop cracking mainly occurred in 2 and 3, 1 and 11, as well as 5 and 6 between amino acid residues. This rule can be applied to cyclosporine cracking rapid structural identification of compounds. Keywords: electrospray ionization multi-stage mass spectrometry cyclosporin structure identification Structural characterization of cyclosporins by electrospray ionization mass spectrometry ABSTRACT Electrospray ionization mass spectrometry method was applied to the structural characterization of cyclosporins, the fragmentation patterns and structural assignments of cyclosporins were discussed. Under the experimental conditions, cyclosporins first form [MH] ion by absorbing a proton on the N atom, then the amide bond split and a series of fragments formed. The main fragmentation pathways started by primary splitting between amino acid residues 2  3,1  11 and 5  6. The corresponding N  terminal b  type ions were common fragment types in the mass spectra . The fragmentation scheme could be used for rapid amino acid sequence determination of cyclosporins. KEY WORDS Electrospray ionization; Mass spectrometry; Cyclosporins; Structural characterization Cyclosporine is a group of fungi produce cyclic 11-peptide substances, among which cyclosporin A (CsA, Fig.1) has been as an important immunosuppressant used in orga