Elevated blood ammonia on spatial learning and memory ability.docVIP

 PAGE \* MERGEFORMAT 7 Elevated blood ammonia on spatial learning and memory ability Study: Pan Chen, Pan Chen-chen, Jinling Xiang, Chen Songfang, Ping Chen [Keywords:] Ammonia; water maze; learning and memory Ammonia poisoning theory put forward in the last century, in the pathogenesis of hepatic encephalopathy play an important role [1]. A large number of studies have shown that hyperammonemia have toxic effects on the central nervous system [2], but its effect on cognitive function of rarely reported. the present study, the rat model of high blood ammonia observed hyperammonemia on spatial learning and memory function, are as follows. 1 Materials and methods 1.1 The main material abundance of ammonium chloride reagent by the Wuxi plant to provide the public (batch number:. 1.2 SD rats fed animal group and 40 from the Animal Center of Wenzhou Medical College (license number: SCXK Zhejiang 2005-0019), weight 200 ~ 240 g, were randomly divided into A group (control group 20) and B group (high blood ammonia model group 20). All rats were constant at room temperature (20 ~ 25 ) and light circadian rhythm control (8:00-20:00), the clean feed, free food, water. 1.3 Methods A: 1 day per week 5 d before the intraperitoneal injection of saline 0.5 mL/100 g, for 4 weeks. B group: ammonium chloride dissolved in saline to a final concentration of 5%, according to 0.5 mL/100 g dose of intraperitoneal injection once a week 1 day ago 5 d for 4 Zhou Jianli chronic hyperammonemia model [3]. 1.4 Morris water maze, Morris water maze test set see [4]. Before the experiment the rats weighed. The experiment was 5 d. 1 free swim days for rats 2 min, starting from the first 2 days, every day in the morning, afternoon, two, each training 4 times, rats in order for the wall into the water from the four points into the pool, climb the platform to find and observe the situation in rats. Every time rats reach the platform, in the 30 s. platform resting o