Eliminate goiter in the preparation of serum containing the determination of harpagoside.docVIP

 PAGE \* MERGEFORMAT 12 Eliminate goiter in the preparation of serum containing the determination of harpagoside Of: Zhangqiu Yan, Han Cui Ming, Liu Ximing, Zhu Xiaoyun, pigeons [Abstract] Objective To develop a swelling consumer preparation and consumption goiter after oral administration in serum Determination harpagoside. Methods RP-HPLC method for the determination of the content of harpagoside, column: Kromasil C18 column ( 250 mm 4.6 mm, 5 m, and Easyguard guard column with acetonitrile and 1% acetic acid solution as mobile phase, gradient elution, flow rate: 1 ml min-1, the detection wavelength was 278 nm, injection volume: 5 l . Results harpagoside in 1.46 ~ 146 g ml-1 concentration range of good linearity, low, medium and high concentrations of harpagoside precision (RSD (n = 5) were 1.05%, 0.59%, 0.80% , day difference (RSD (n = 5 to 1.00%, preparations and serum harpagoside of recoveries (n = 5, respectively (98.02 + -1.01% and (101.03 + -1.64%, determined reproducibility (RSD (n = 6, respectively, 0.73% and 0.96%, per gram of preparations containing the amount of harpagoside 230.5 g, per ml serum in the amount of harpagoside as 1.88 g. Conclusion The method is simple , rapid, accurate, good reproducibility and can be used in the preparation and serum determination of harpagoside. [Keywords:] harpagoside, a swelling consumer preparation, solid phase extraction, HPLC ChinaAbstract: ObjectiveTo develop a determination method of Harpagoside in Jia Zhong Xiao preparation and blood serum of rat. Methods The RP-HPLC method was applied as with: Kromasil C18 column (250 mm 4.6 mm, 5 m and Easyguard column. The mobile phase was acetonitrile and 1% acetic acid with linearity gradient elution, the flow rate at 1 ml min-1, and detection wavelength was 278nm. ResultsAn excellent linearity was obtained over the range of 1.46 ~ 146 g ml-1. The RSDs of accuracy for 7.3, 29.2, 146g ml-1 were 1.05%, 0.59%, 0.80%, respectively (n = 5). The RSDs of in