ELISA detection of magnetic separation FSH LH and hCG.docVIP

 PAGE \* MERGEFORMAT 8 ELISA detection of magnetic separation FSH LH and hCG [Keywords:] magnetic separation enzyme FSH LH hCG ELISA magnetic separation technology is the mid-80s Swiss Serono (Shi Langluo) invented a non-diagnostic center isotopic immunoassay advanced technology, called magnetic antibody immunoassay (MAIA). The double antibody sandwich ELISA in free type of labeled antibody and labeled antigen - antibody complex with the separation of full and rapid separation of the advantages of magnetic separation technology for creating the conditions for the promotion. We invented by the center double-antibody sandwich enzyme-linked immunosorbent SEROZYME detection system (by enzyme-linked Quantitative determination of endocrine and immune supporting apparatus to form a complete kit for the detection system), Determination FSH, LH and hCG, the test results reported below. 1 Principle Analytes (FSH, LH and hCG), respectively, with alkaline phosphatase (ALP) and fluorescein isothiocyanate (FITC) labeled IgG monoclonal antibody formation. Antigen-FITC labeled antibody complex, the double-antibody sandwich, coupled with magnetic separation agent (combination with and anti-FITC magnetic particles) to combine complex with magnetic particles, attracted by the permanent magnet to bottom of the tube to precipitate, separation HRP, adding substrate phenolphthalein monophosphate (PMP), ALP catalytic PMP release of water to explain phenolphthalein, pH 10.5 buffer solution was pink, then to a permanent magnet to attract magnetic compound precipitation, the supernatant solution set SEROZYME-I quantitative determination of endocrine-type enzyme-linked immunosorbent measured. 2 Materials and methods 2.1 Instrument SEROZYME-I-type endocrine quantitative enzyme-linked immunosorbent analysis (three-wavelength 492 nm, 550 nm, 630 nm at the same time detection, program editing, curve drawing, computer control, print output), magnetic separator and v