ELISA detection of p24 antigen for diagnosis of HIV infection status.docVIP

 PAGE \* MERGEFORMAT 12 ELISA detection of p24 antigen for diagnosis of HIV infection status [Keywords:] ELISA; p24; HIV 1 HIV Research laboratory diagnosis of infection HIV primarily through sexual contact, blood and mother to child transmission. In recent years, the number of infections in less developed countries than in developed countries. Estimated that by 2010 the number of people in China infected with HIV will be more than 1 0 million. Serum HIV antibodies are present diagnosis of HIV infection in the conventional experimental methods, but the determination of HIV antibodies have limitations: There are more than 70% of HIV-infected people infected after 6 months in order to detect antibodies in the gay community, the figure is over 80% , detection technology to increase the HIV ‘window period’ the risk of transmission [1]; newborns need to produce antibodies one year after birth from the mother’s HIV antibodies produced false-positive result; HIV antibodies persist, disappear when the last stages of AIDS can not be stability index as a treatment for monitoring. 2 p24 and experimental value of the source 24 kDa protein p24 is the HIV virus-like particles of the major structural protein in packaging and maturation of the virus play an important role. amino acid sequence of p24 protein in the HIV highly conserved among different strains, lack of p24 will lead to the virus can not be properly assembled. Capsid protein, p24-specific very strong, and most other retroviruses no cross-reaction, can be used for early diagnosis of HIV infection, anti-HIV drug efficacy testing and the HIV infection progressed to AIDS dynamic observation. HIV  1 infection in the body, the infection first appeared in the blood is a virus gag gene products p24 antigen, and then a corresponding specificity of anti-HIV antibodies, with antibody titers after the increase of p24 antigen content was a gradual downward trend , as well as undetectable. In the late progressio