ELISA combined toluidine red unheated serum test for detection of Treponema pallidum infection of the Effect of.docVIP

 PAGE \* MERGEFORMAT 8 ELISA combined toluidine red unheated serum test for detection of Treponema pallidum infection of the Effect of Of: Zhou Liang, Li Jie, Zhou Songwei, Chen [Keywords:] syphilis; detection; enzyme-linked immunosorbent assay; toluidine red unheated serum test Treponema pallidum gelatin particle agglutination test (TPPA method) is a recognized confirmatory test for syphilis-specific antibodies, but the method is difficult to achieve the “three” of automation, standardization and normalization, data are difficult to save. Currently blood banks and hospital transfusion department detection of Treponema pallidum antibodies by ELISA method and more. the sensitivity and specificity are high, and the TPPA was no significant difference between methods [1], can realize “three”, information is also easy to preserve. but positive for syphilis antibody ELISA method, still can not decide whether the current infection, treatment can not be the basis for the doctors. toluidine red unheated serum test (TRUST method) there is a big missed rate [2], blood banks and hospital transfusion department does not separately applied to the detection of syphilis, but TRUST assay could supplement judged as positive for current infection, the basis for doctors to provide treatment. are many blood banks have also adopted laws TRUST ELISA and recheck the early detection of blood donors [3-5], the two methods complement each other, the sensitivity and specificity of the method with the TPPA, and played out the advantages of ELISA method. We have since 2004-2005 with ELISA, TRUST, TPPA three of 385 cases of syphilis detection positive samples were compared to 2008 and .2006 by ELISA assay method combined TRUST 7656 required blood transfusion cases, STD clinic clients were 355 cases, a total of 8011 specimens, the results reported below. 1 Materials and methods 1.1 The source of specimen There are specimens of the two periods: from January 2004 to Decembe