Menispermi extract the establishment of an effective component analysis.doc

 PAGE \* MERGEFORMAT 13 Menispermi extract the establishment of an effective component analysis Author: Zhu Xiaoqin, Han Tie-Gang, Zhang Huan, Liu Cuizhe [Abstract] Objective acid dye colorimetry Menispermi extract Menispermi alkaloids (TAMD) content, using high performance liquid chromatography (HPLC) determination of dauricine (Dauricine) content. Bromocresol green method in anhydrous methanol (pH = 6), you can direct complexation with the TAMD generate green compounds, for colorimetric determination of the maximum absorption wavelength of 620 nm. HPLC, column: Discovery C18 column; mobile phase: acetonitrile: water: phosphoric acid = 16:84:0.15; flow rate: 0.5 ml * min-1; detection wavelength: 208 nm. The results of acid dye colorimetry: The linear range of 6 ~ 40 μ g * ml-1, r = 0.999 8 (n = 7), the average recovery was 99.18%, RSD = 0.74%; HPLC method: The linear range of 0.01 ~ 0.1 mg * ml-1, r = 0.999 8 (n = 7), the average recovery was 98.32%, RSD = 0.94%. Conclusion The method is sensitive, accurate, reproducible, can be used as Menispermi extract TAMD and dauricine content analysis method. [Keywords:] Bat Pueraria Menispermi alkaloids acid dye colorimetry high performance liquid chromatography Abstract: ObjectiveTo establish an acid dye colorimetric method for determination of total alkaloid in Menispermum dauricum (TAMD), an HPLC method for determination of Dauricine. MethodsTAMD produced green combination with bromocresol green in the condition of dehydrated ethanol, according to this characteristic, acid dye colorimetry was established. HPLC method: The separation was carried out on a Discovery-C18 column (250 mm * 4.6 mm, 5 μ m), the mobile phase was acetonitrile-water-phosphoric acid (16:84:0.15), the flow rate was 0.5 ml * min-1, and detection wavelength was 208 nm. ResultsAcid dye colorimetric method: The linear range was 6 ~ 40 μ g * ml-1, r = 0.999 8 (n = 7), the average recovery was 99.18% with the RSD of 0.74%. HPLC method: The lin