Metabonomics and its progress in research in the field of microbial.doc

 PAGE \* MERGEFORMAT 9 Metabonomics and its progress in research in the field of microbial Paper Keywords: metabonomics Environmental Microbiology Reviews Abstract: Metabolomics is modeled genomics and proteomics research thinking on the living body quantitative analysis of all metabolites and look for metabolites, and physiological and pathological changes in the relative relationship of research. This paper introduces the basic meaning of metabolomics based on, for metabonomics research methodology and its application in environmental microbiology research in the field of progress have been reviewed. First, an overview of microbial metabolism Metabolomics (metabonomics / metabolomics) is to follow the example of genomics and proteomics research thinking on the living body quantitative analysis of all metabolites and look for metabolites, and physiological and pathological changes in the relative relationship of research is a systems biology components. The object of their research are mostly less than 1000 molecular weight small molecules. Combination of advanced analytical techniques such as pattern recognition and expert system calculation and analysis is the study of metabonomics basic methods. Chemical analysis techniques most commonly used is the 1H nuclear magnetic resonance (1HNMR) and chromatography (capillary electrophoresis) - mass spectrometry (X-MS). Metabolomics data processing is currently the main methods are: application of principal component analysis (PCA), etc. from the original map information or classified information after pretreatment and using the appropriate visualization technology to express visually; the establishment of inter-class mathematical model, so that all kinds of maximum separation between the sample and take advantage of the establishment of multi-parameter model to predict the unknown samples; the eventual establishment of the field can make use of the application of database and expert systems. Application of metab