Semi-nested methylation-specific polymerase chain reaction on the tumor cell lines or deletions of p15 gene methylation status of the detection.docVIP

 PAGE \* MERGEFORMAT 21 Semi-nested methylation-specific polymerase chain reaction on the tumor cell lines or deletions of p15 gene methylation status of the detection Author: Lin Fuan YE Bao-Guo Shen Jian-zhen Zhou Huarong FU Hai-Ying Fan Liping Abstract This study analyzes a variety of cancer cell lines or deletions of p15 gene methylation status, to clarify or deletions of p15 gene methylation in tumor genesis and development role. The use of semi-nested methylation-specific polymerase chain reaction (hemi  nested methylation specific polymerase chain reaction, hn  MSP) analysis of 20 kinds of cancer cell lines and normal human mononuclear cells or cell lines of the p15 gene methylation and missing status, and to evaluate the sensitivity and specificity of the method. The results showed that: In all cells, Molt  4, Raji, KG1, CA46, SW480, NCE, SMMC  7221, NCI  H446 cells partially methylated, hn  MSP detection of p15 gene methylation-sensitive can be achieved 1 × 10-5. Normal mononuclear cells, HL  60, HeLa, HepG2, 293, SGC7901, U266, CEM cells, compared with non-methylation of p15, and K562, NB4, GMC, Jurkat seems to show p15 gene deletion or mutation. Conclusion: p15 gene methylation or deletion in a variety of tumors, especially in hematologic malignancies have a higher incidence of, and the progress of the disease and prognosis are closely related, hn  MSP has a high sensitivity and specificity of nature, it is worth the clinic. Keywords: p15INK4B; gene methylation; gene deletion; hn  MSP Detection of p15 Gene Methylation or Deletion Status in Different Malignant Cell Lines by Using Hemi  nested Methylation Specific Poly  merase Chain Reaction Abstract This study was aimed to investigate the methylation or deletion status of p15 gene in different malignant cell lines, and further to clarify their roles in the development and progression of malignant tumors. Hemi  nested methylation specific polymerase chain r