SEREX method in screening and identification of antigens of human osteosarcoma.docVIP

 PAGE \* MERGEFORMAT 22 SEREX method in screening and identification of antigens of human osteosarcoma Keywords: SEREX method osteosarcoma; antigen 0 Introduction Tumor antigen and its encoding gene of the theoretical study and experimental technology allows screening of tumor antigens and tumor immunity research into a new phase [1]. Screening and identification of tumor antigen-specific immunotherapy is a prerequisite for the clinical and key, with tumor biology and tumor immunology in-depth understanding of the design of humoral immunity based on the reorganization of the serological analysis of cDNA library (serological analysis of recombinant cDNA expression libraries, SEREX) method, is easy to [2]. In this paper, SEREX method principles, technical steps, and its genetic screening in osteosarcoma tumor status of the application and research to make a brief introduction. Principle 1 SEREX method Recombinant cDNA expression library was first proposed by the serological analysis of Pfreundsch team building [2]. SEREX method of molecular cloning techniques and the use of autologous serum in patients with autologous tumor cells, integration of sub-technologies, not only can detect antibody response, but also in the antigen and autologous serum in patients with reactions, based on the molecular level to determine directly from the original immune of tumor protein (antigen). The process of this method mainly of fresh tumor tissue or cell sample cDNA library to build and connect into the phage expression vector, in order to re-transfected E. coli phage of E. coli, the bacterial expression of recombinant proteins transferred to NC membrane, and diluted patients in autologous serum were incubated with enzyme-linked specific anti-human IgG secondary antibody identification and high-titer of serum antibody response of the cloning, subcloning of positive clones were subsequently isolated a single insert and to identify the insert