Sol-gel-based film and multi-walled carbon nanotubes - Pt nanoparticles efficiency of the current type L-lactic acid biosensor.docVIP

 PAGE \* MERGEFORMAT 17 Sol-gel-based film and multi-walled carbon nanotubes / Pt nanoparticles efficiency of the current type L-lactic acid biosensor OF: He Xiao Jinghua Ge core in the Optical Zhang Xiuming Lin Qing Shen Shuo Yuan Feng Zhu Han Dong Liang Huang [Abstract] was constructed based on multi-walled carbon nanotubes (Multi-walled carbon nanotubes, MWCNTs and Pt nanoparticles (Pt-nano’s current type L-lactic acid biosensor. The Sol-gel membrane covering the L-lactate oxidase (L -lactate oxidase, LOD and MWCNTs / Pt-nano-modified electrode surface. The experimental results show that: the best working conditions for the sensor: Detection voltage 0.5 V, buffer, pH 6.4, test temperature 25 ℃. The sensor response time is 5 s, sensitivity is 6.36 μA / (mmol / L. Continuous testing 4 weeks 90% of its activity remained linear range is 0.2 ~ 2.0 mmol / L, and anti-interference ability. in the actual testing of blood samples, this sensor and traditional spectrophotometry has a good consistency. [Keywords:] biosensor, L-lactic acid, sol - gel, platinum nanoparticles, multi-walled carbon nanotubes Abstract An electrochemical L-lactate biosensor was fabricated by combining Platinum nanoparticles (Pt-nano) with multi-walled carbon nanotubes (MWCNTs). L-lactate oxidase (LOD) was immobilized on the surface of the glassy carbon electrode (GCE) modified with MWCNTs and Pt-nano.The surface of resulting LOD / MWCNTs / Pt-nano electrode was covered by a thin layer of sol-gel to avoid the loss of LOD and to improve the anti-interference ability.The cyclic voltammetric results indicated that MWCNTs / Pt-nano catalyst displayed a higher performance than MWCNTs.Under the optimized conditions, ie, applied potential of 0.5 V, pH 6.4,25 ℃, the proposed biosensor’s determination range was 0.2-2.0 mmol / L, response time was within 5 s , and the sensitivity was 6.36 μA / (mmol / L.It still kept 90% activity after 4 weeks.The fabricated biosensor had practically good sel