Soothe the nerves of brain fluid quality standards.docVIP

Soothe the nerves of brain fluid quality standards Study: Ancient sea front, Amanda Zhang Ran Liu, Guo Zhu [Abstract] Objective To soothe the nerves of brain fluid quality standards. Methods TLC identification of Radix, Salvia, wolfberry and ginseng; by high performance liquid chromatography Schisandrin Schisandra content, C18 (250 mm × 4.6 mm, 5 μm) column, mobile phase was methanol - water (55:45), flow rate 1.0 mL / min, detection wavelength 250 nm. The results can be detected by TLC Salvia, wolfberry and ginseng characteristic spots; schisandrin in the range 0.121 8 ~ 1.218 μg good linear relationship, r = 0.999 9, precision RSD = 0.22% (n = 6), reproducibility RSD = 1.1% (n = 6), the average recovery was 97.34% (RSD = 1.7%, n = 6). Conclusion The method is simple, sensitive, specific, reproducible, and can soothe the nerves of brain fluid as a quality control method. [Keywords:] nerves brain fluid; schisandrin; performance liquid chromatography; thin layer chromatography; quality standards Abstract: Objective To study the quality standard of Anshen Jiannao Mixture. Methods Radix ophiopgonis, Radix et Rhizoma Salviae Miltiorrhizae, Fructus Lycii and Radix et Rhizoma Ginseng were identified by TLC. HPLC was used to determine the content of Schizandrol A on C18 (250 mm × 4.6 mm, 5 μm) ODS column. The mobile phase was consisted of methanol-water (55:45). The flow rate was 1.0 mL / min. The detection wavelength was at 250 nm. Results The characteristic spot of Radix et Rhizoma Salviae Miltiorrhizae, Fructus Lycii and Radix et Rhizoma Ginseng could be easily examined by TLC. The calibration curve of Schizandrol A was linear in the range of 0.121 8 ~ 1.218 μg, r = 0.999 9. The precision RSD was 0.22% (n = 6 ). The reproducibility RSD was 1.1% (n = 6). The average recovery rate of Schizandrol A was 97.34% (RSD = 1.7%, n = 6). Conclusions The method is easy, sensitive and specific with good reproducibility. It can be used to control the quality of Anshen J