Tetrazine Diformamide vitro NB4 cell proliferation apoptosis and differentiation in vitro.docVIP

 PAGE \* MERGEFORMAT 30 Tetrazine Diformamide vitro NB4 cell proliferation apoptosis and differentiation in vitro Author: Zhou columns, Ya-Ping Lü, Wei-Xiao Hu, Lian-Nü Qiu, Wen-Song Wang, Wu, Liu Jiandong 【Abstract】 In order to observe the tetrazine-dimethyl amide (ZGDHu-1) on the NB4 leukemia cell line proliferation, differentiation and apoptosis, and its role in the mechanism discussed. Different concentrations of ZGDHu-1 and NB4 cells in vitro, and to all-trans retinoic acid as positive drug controls were observed ZGDHu-1 Dui NB4 cell proliferation, apoptosis and differentiation. Analyzed by flow cytometry ZGDHu-1-induced NB4 cell differentiation and apoptosis during the bcl-2 and bax expression changes, and P38MAPK and STAT3 phosphorylation levels, while taking advantage of real-time fluorescence quantitative PCR detection of telomerase hTERT-mRNA expression in change. The results showed that: ZGDHu-1 Dui NB4 cell proliferation and viability in a time-and dose-inhibition dose-effect relationship, 48 and 72-hour IC50, respectively 450 and 200 ng / ml. NB4 cells treated with ZGDHu-1 effect, the majority of cells were arrested at the G2-M phase, G0 / 1 phase cells decreased; cells have the typical morphological changes, DNA fragmentation, sub-G1 peak detected and significantly increased, Annexin-V / PI marked increase; Hoechst33258 see apoptotic cells after fluorescence staining of the characteristic changes, and these are confirmed to be ZGDHu-1 could induce NB4 cell apoptosis. NB4 cells were treated with 2-100 ng / ml ZGDHu-1 role in three days, the cells partially differentiated, NBT-positive rate of increase in cell surface CD11b, CD13 expression increased. ZGDHu-1 role in NB4 cells, bcl-2 expression remained unchanged, but the expression of bax increased significantly, bax/bcl-2 ratio increased phosphorylation P38MAPK expression increased, while no change in phospho-STAT3 expression and telomerase hTERT - mRNA expression with the role of ZG