Thapsigargin on human lens epithelial cell line growth inhibition mechanism of SRA01-04.docVIP

 PAGE \* MERGEFORMAT 24 Thapsigargin on human lens epithelial cell line growth inhibition mechanism of SRA01/04 Author: Ji-Hong-pei, Wu star, Peng Ruiping 【Abstract】 【Objective】 thapsigargin (TG) on cultured human lens epithelial cell line SRA01/04 proliferation, and to explore its molecular mechanism. 【Methods】 different concentrations of TG treatment SRA01/04 cells, 72 h, MTT assay (MTT) test cell proliferation; flow cytometry (PI method) to detect changes of cell cycle; transmission electron microscopy ultrastructural changes of the cells were observed; TUNEL Detection of DNA breaks in nuclei situation; laser confocal microscopy and flow cytometry Caspase3 active subunit (17/19 ku) expression and expression of positive cells. 【Results】 MTT measured by TG is 0.325 ~ 5 μmol / L, the cell proliferation inhibition rate changed significantly, IC50 of about 0.8 μmol / L; Transmission electron microscopy revealed cell membrane TG treatment SRA01/04 shrinkage, chromosome condensation and margination , nuclear membrane lysis, apoptotic body formation; flow cytometry (PI method) and the TUNEL assay TG concentration 0,0.1,0.5,1,10 μmol / L treated separately SRA01/04 cells, 72 h, apoptosis rate increases with the concentration of a dose-dependent; TG treated cells also showed positive expression rate of Caspase3 concentration-dependent increase, TG of 10 μmol / L, almost every cell cytoplasm Caspase3 have been activated. 【Conclusion】 Thapsigargin may be induced by activation of Caspase3 for cultured human lens epithelial cell line SRA01/04 apoptosis and inhibit its proliferation, TG for the prevention of after-cataract drug research provides the basis. Keywords: Thapsigarigin; lens epithelial cells; apoptosis; Caspase3 Effect and Mechanism of Thapsigargin on Proliferation of Human Lens Abstract: 【Objective】 To investigate the effect of thapsigargin (TG) on the proliferation of human lens epithelial cells line SRA01/04 in vitro and under