The body to build bone TFCC repair osteochondral defects in rabbits.docVIP

 PAGE \* MERGEFORMAT 23 The body to build bone TFCC repair osteochondral defects in rabbits Abstract of cartilage tissue engineering approach to repair bone cartilage defects in the ideal seed cells and scaffold materials. [Methods] In vitro induction of bone marrow stromal cells to the directional differentiation of chondrocytes were cultured with PLGA scaffold, and to imitate mosaic osteochondral grafting in canine articular cartilage defects by deep-MSC-stent-graft implantation, shallow induction into cultured The MSC-scaffold, close-voltage distribution, the body to build bone cartilage complex, to observe the repair situation. [Results] 16-week experimental group, entirely hyaline cartilage defect repair, fully integrated with the surrounding normal cartilage, organize observations indicate that in cartilage cells to repair the main subchondral bone formation, tidal lines of basic restoration; negative control group, mainly by the fiber defect tissue repair, in part by the hyaline cartilage repair, organize observations indicate that cellular repair with fiber-based, mixed with some cartilage cells; blank control group entirely by fibrous tissue repair. [Conclusion] MSCs via the directional differentiation of chondrocytes after the PLGA composite scaffold, through the close press-fit manner MSCs-PLGA stent in the body to build bone cartilage complex, can be effectively repaired osteochondral defects. Keywords: bone marrow mesenchymal stem cells transplantation mosaic osteochondral composite bone cartilage defects Abstract: [Objective] To evaluate the effect of treating the osteochondral defects with implanted cell  scaffold composites, cultured MSCs as seed cells and PLGA as scaffolds, and to acquire desirable seed cells and scaffold materials. [Method] BMSCs were induced to differenciatiated into chondrocytes, co  cultured with PLGA scaffold respectively in vitro, then implanted into osteochondral defects on canine models by usin