Tight junction proteins occludin and ZO1 in guinea pig cochlea lateral wall of blood vessels in the expression pattern.docVIP

 PAGE \* MERGEFORMAT 13 Tight junction proteins occludin and ZO1 in guinea pig cochlea lateral wall of blood vessels in the expression pattern [Abstract] Objective: To study the tight junction protein occludin, ZO  1 in guinea pig cochlea lateral wall of blood vessels in the expression patterns. Methods: Healthy adult guinea pigs versicolor, auricular reflex sensitivity, were collected from the cochlea, brain, lung three groups of organizations, immunohistochemistry and Western Blot Study of tight junction proteins occludin, ZO  1 in the cochlea lateral wall of blood vessels in the expression pattern to the brain group, the lung as a positive control group. Results: light microscope, the lateral cochlear wall stria vascularis marginal cells such as capillary endothelial cells were strongly positive granules in the expression of the brain group, pulmonary capillary endothelial cells lining group also showed significant expression; Western Blot under occludin, ZO  1 high expression in the cochlea of the group, and the apparent higher than the brain group and the lung group. Conclusion: occludin, ZO  1 in guinea pig cochlea lateral wall of blood vessel patterns in the high concentration of tight junction expression, and maintenance of blood labyrinth barrier structure and function are closely linked. [Keywords:] occludin protein; ZO  1 protein; connected proteins; cochlea; blood labyrinth barrier 0 Introduction Blood labyrinth barrier (blood labyrinth barrier, BLB) guarantees the existence of a relatively stable micro-environment of the inner ear to maintain the normal physiological function of the inner ear. It is mainly from the cochlear lateral wall, stria vascularis, spiral ligament at a continuous windowless capillary endothelial cells to form [1]. stria vascularis of cochlear duct membranes constitute an important component of the outer wall by the capillary and marginal cells, intermediate cells and basal cells. capillary basement membrane mea