Time-resolved fluorescence analysis of signal amplification technology research progress and application of.docVIP

 PAGE \* MERGEFORMAT 24 Time-resolved fluorescence analysis of signal amplification technology research progress and application of [Abstract] Time-resolved fluorescence signal amplification technology is a new type of micro amount of marker analysis techniques. It is based on biological signal amplification-based, lanthanide chelate as a marker in order to time-resolved and wavelength-resolved method for measuring the full use of the enzyme marker analysis and PCR amplification analysis techniques such as the advantages of technology. Its unique advantages of non-isotopic and ultra-sensitivity. This review describes the characteristics of TRFA enzyme amplification, the second enzyme amplification, Eu nanoparticles, rolling circle amplification, immunization PCR and fluorescence quenching analysis, signal amplification, time-resolved fluorescence analysis technique research and application. Time-resolved fluorescence analysis of signal amplification technology in the biological analysis of an extremely wide range of applications, showing a good development prospects. [Keywords:] signal amplification; time-resolved fluorescence analysis; lanthanide chelate Studies and applications of time-resolved fluorescence assay based on signal amplification [Abstract] Time-resolved fluorescence assies (TRFA) based on signal amplification are new ultrasensitive labelling assay technologies.The basis is biosignal amplification, the labels are lanthanide chelates, and the detection methods are the time-resolved and wavelength-resolved fluorescence assay in the technologies.In addition, enzyme-labelled assay and PCR amplification et al are used in the technologies.The several main signal amplification methods and amplifications, for example, enzyme-amplification, two-round enzyme-amplification, europium nanopartical, rolling circle amplification , immuno-PCR and fluorescence quenching assay et al, were introduced in the summary. The unique advantages of nonisotope and