Tissue microarrays to detect CD31 CD105 v-WF PCNA expression in HCC tissues.docVIP

 PAGE \* MERGEFORMAT 23 Tissue microarrays to detect CD31 CD105 v-WF PCNA expression in HCC tissues Abstract [Objective] To investigate the MVD-CD31, MVD-CD105, MVD-v-WF and PCNA expression in association with the biological behavior of hepatocellular carcinoma. [Methods] construct contains 38 cases of hepatocellular carcinoma and adjacent tissues of the tissue microarray, immunohistochemical method to detect MVD-CD31, MVD-CD105, MVD-v-WF and PCNA expression in the same period, double immunofluorescence staining Detection of CD31 and PCNA expression. [Results] The tumor tissue MVD-CD31 mean 48.5 ± 29.7, while the adjacent tissues MVD-CD31 mean 24.2 ± 22.3, the difference was significant (P lt;0.01); PCNA expression in tumor tissue of high MVD-CD31 mean ( 68.3 ± 36.9) was significantly higher than the low PCNA expression in HCC tissues (37.9 ± 30.9, P = 0.012). MVD-CD105 and MVD-v-WF in the HCC tissue and adjacent tissues were 22.4 ± 12.3, and 15.9 ± 11.7; 21.5 ± 13.3, and 34.0 ± 16.7, difference was not significant. Existence of intrahepatic metastasis of HCC tissue MVD-CD105 mean 28.4 ± 19.4, without intrahepatic metastasis of the tumor tissue MVD-CD105 mean 5.4 ± 5.2, the difference was significant (P lt;0.01); there of intrahepatic metastasis of the hepatocellular carcinoma and non-liver metastasis tumor tissue MVD-CD31 and MVD-v-WF, respectively 66.6 ± 39.4, and 44.9 ± 32.9; 25.2 ± 15.2, and 19.7 ± 9.9, difference was not significant. [Conclusion] MVD-CD31 and the proliferation of state-related hepatocellular carcinoma, MVD-CD105 and intrahepatic metastasis of liver cancer, tumor angiogenesis may occur in the liver plays an important role in the evolution. Keywords: liver neoplasms Angiogenesis of tumor growth and metastasis of the premise, at present, as a variety of tumor prognostic marker of tumor microvessel density (microvessel density, MVD) received extensive attention [1]. Dye molecules through the endothelial cell marker refl