TMP on different brain regions after cerebral ischemia of neuronal NO synthase expression.docVIP

 PAGE \* MERGEFORMAT 23 TMP on different brain regions after cerebral ischemia of neuronal NO synthase expression Of: Qiu Fen, Liu Yong, Ma Bo, Qi kept Fang, Wang Wenjing [Abstract] Objective To observe the different time after cerebral ischemia in different brain regions of neuronal NO synthase (nNOS) expression of TMP on the expression of nNOS after cerebral ischemia. Methods Adult male SD rats were randomly divided sham group, model group and the TMP low (20mg/kg), in (40mg/kg), high (80mg/kg) dose to 5 groups according to ischemic time was divided into 1,3 , 7,14,21 d 5 subgroups. patching method in the left middle cerebral artery occlusion model, 2h after intraperitoneal injection of different doses of TMP (1 / d), to death before the 2h. immunohistochemical staining lateral ventricle at different times after cerebral ischemia subventricular zone (SVZ), corpus callosum (CC), infarct area around the striatum and cerebral cortex, hippocampus CA1 area and dentate gyrus (DG) nNOS expression. Results sham group brain regions nNOS expression at different times are similar, the difference was not statistically significant (Pgt; 0.05). In the SVZ, the model group 1 ~ 14d nNOS expression decreased, 21d when the higher dose groups showed TMP 3 ~ 14d nNOS expression was significantly lower , 21d significantly increased. In CC, the model group significantly decreased 3 ~ 14d, 21d has gone up, Xiong triazine nNOS expression in each dose group showed significantly lower for the 3 ~ 14d to the high dose group decreased most significantly, 21d increased. In the ischemic cortex and the striatum, nNOS expression in the model group showed significantly lower for the 3,7 d, 14,21 d increase significantly in TMP nNOS expression in each dose group 3 ~ 21d are low, medium, high lower dose group, the most obvious 7 ~ 14d, 21d when the slight rebound. In the DG, CA1 area, model 3,7 d nNOS expression was significantly reduced, 14d when the higher dose groups TMP 3 ~ 21d nNOS