Tumor necrosis factor-α on cultured bovine trabecular meshwork cells MMP3 MMP9 and TIMP-2 expression in.docVIP

 PAGE \* MERGEFORMAT 21 Tumor necrosis factor-α on cultured bovine trabecular meshwork cells MMP3 MMP9 and TIMP-2 expression in Author: Wu Qiong, Dexiu, Li Chen, Qi Xiangyun 【Abstract】 Objective: To observe the tumor necrosis factor-α on cultured bovine trabecular meshwork cells, MMP3, MMP9, and TIMP-2 expression, and to explore primary open-angle glaucoma pathogenesis. Methods: Primary bovine trabecular meshwork cells and subcultured, using immunohistochemical methods (NSE, Ⅷ factor related antigen staining) to identify cells, chemical and electronic transmission electron microscopy on cell morphology and growth characteristics of the observation; right Chuan-3 generation of trabecular meshwork cells with TNF-α were to impose a final concentration of 0,12.5,25,50 μg / L of culture medium, 48h Across MMP3 and MMP9 immunohistochemical SP staining, the results of computer image analysis and were statistically test. Grouping extracted cell culture medium with ELASA detected changes in the amount of TIMP-2. Results: In vitro cultured bovine trabecular meshwork cells express MMP3 and MMP9, TNF-α can increase the expression of MMP3 and MMP9, and inhibit the expression of TIMP-2. Conclusion: TNF-α in a certain range can increase the MMP3 and MMP9 expression (P lt;0.05), and inhibit the TIMP-2 expression (P lt;0.01), so TNF-α can reduce the trabecular meshwork cells, extracellular matrix accumulation, so that aqueous drainage unobstructed, for the surgical laser trabeculoplasty theory description of a certain significance. Keywords: bovine trabecular meshwork cells 0 Introduction ECM (extracellular matrix, ECM) is the abnormal accumulation of primary open-angle glaucoma (primary open angle glaucoma, POAG) trabecular meshwork organization important change. Matrix metalloproteinases (matrix metalloproteinases, MMPs) are a family of zinc protease degradation of ECM components type, for maintaining the continuous generation and degradatio