Turtle cooked material before and after the effective parts of hepatic fibrosis Comparison of HPCE Fingerprinting_0.docVIP

 PAGE \* MERGEFORMAT 16 Turtle cooked material before and after the effective parts of hepatic fibrosis Comparison of HPCE Fingerprinting Of: Shi Jing Ni, Chen Jinwen, Goh Rong, Tang Yin Ping, Hu Chunling, Zhou Li, Wen Yan Liu [Abstract] Objective To establish a Health and vinegar turtle turtle parts of active substances on hepatic fibrosis by Capillary Electrophoresis (HPCE) fingerprinting to investigate the efficacy of substances before and after processing the difference. Methods Using capillary zone electrophoresis, 75 μm × 57 cm uncoated silica capillary, running buffer was 20 mmol / L disodium hydrogen phosphate -100 mmol / L Tris aminomethane (l mol / L NaOH to adjust pH value of 12.0), separation voltage 18 kV, The detection wavelength was 254 nm, column temperature was 20 ℃, 10 batches of students were established, and 10 batches of vinegar turtle turtle effective parts of the fingerprint material, and were compared. The results were calibrated raw turtle fingerprints of 17 total Feng, HPCE pattern similarity greater than 0.94, vinegar turtle fingerprints were 19 calibration peaks, HPCE pattern similarity were greater than 0.93, and 10 batches of raw turtle shell pattern and vinegar were Fingerprint of turtle decreased from 0.86 to 0.92, and 10 batches of vinegar and raw turtle turtle pattern similarity of fingerprints were also reduced from 0.86 to 0.93. Conclusion turtle chemical composition and its content before and after processing has changed, and the processing resulting from a number of new active ingredients. [Keywords:] turtle, processing, active ingredients, high performance capillary electrophoresis, liver fibrosis, Fingerprint Abstract: Objective A fingerprint method was established for comparing the same geographical origin of pre-and-post-processed Trionyx sinensis carapace in anti-liver fibrosis, 10 crude samples and 10 vinegar-processed were analyzed. Method Using HPCE detection method equipped with uncoated fused silica ca