Two tumor cell lines HLAI Molecular Identification of gene expression and significance of.docVIP

 PAGE \* MERGEFORMAT 16 Two tumor cell lines HLAI Molecular Identification of gene expression and significance of Author: Kwan Tak-ying, Shen Han, Shao Hongwei, Huang Shulin [Abstract] Objective To study the two common tumor cell lines Hela cells and BEL  7402 cells, class I molecules HLA  I gene expression level and impact of the possible implications of CTL responses. Of tumor cells to extract genomic DNA, using PCR reaction of genomic DNA level identification of class I molecules HLA  I gene copy number situation; extracted total cellular RNA, RT  PCR synthesized cDNA, using PCR reaction identification of tumor cell HLA  I class I molecules in RNA expression levels. The results of two tumor cell HLA  I genes in the genome copy number levels decreased significantly; in RNA level, Hela cells, HLA  A, HLA  B, and HLA  C genes were expressed, the expression level decreased significantly , BEL  7402 cells in HLA  A and HLA  C gene expression, but the HLA  C to reduce the level of gene expression, but no significant HLA  B gene expression. Conclusions RNA level, some class I molecules HLA  I gene expression in reduced or absence of speculation may have contributed to BEL  7402 cells, Hela cells were CTL responses than the decline of one of the reasons. [Keywords:] HLA  I class I molecules; Hela cells; BEL  7402 cells; mRNA Abstract: Objective To investigate the expression level of HLA  I molecule genes in two tumor cell lines, Hela and BEL  7402 and the possible significance of CTL reactions.Methods Genome DNA were extracted from these two tumor lines for detecting the HLA  I molecule genes’ levels by PCR.Total RNA from two cell lines were extracted for RT  PCR, HLA  I molecule genes’expression on RNA level were judged by PCR and agarose gel electrophoresis.Results HLA  I molecule genes levels did not decrease significantly on genome level.On RNA level, HLA  A, HLA  B and HLA  C all expressed normally witho