Umbilical cord blood-derived dendritic cells T cells to promote tumor cell-killing effect of.docVIP

 PAGE \* MERGEFORMAT 31 Umbilical cord blood-derived dendritic cells T cells to promote tumor cell-killing effect of Author: Qu Xin-dong Qu Zheng-hai, left Jianxin, Li-Rong Sun Abstract This study investigated the human umbilical cord blood mononuclear cells in vitro induced differentiation of dendritic cells (DC) to promote T-cell tumor cell-killing effect. The use of lymphocyte separation medium density gradient centrifugation to obtain cord blood mononuclear cells by adhesion method to obtain adherent cord blood mononuclear cells by granulocyte  macrophage colony-stimulating factor (GM  CSF), interleukin  4 (IL  4) In vitro differentiation of cord blood DC. By indirect immunofluorescence assay DC, MTT assay DC-activated T cells to tumor cell-killing effect. Test group plus DC, DC and the proportion of tumor cells, respectively 20:1,50:1,100:1 the control group without DC. The results showed that: cord blood mononuclear cells induced by GM  CSF, IL  4 after induction, the first 15 days can be seen in the inverted microscope the typical morphology of DC. Fluorescence microscope, CD1a cell rate (43.12 ± 5.83)%. The experimental group and control group, experimental group DC to stimulate T-cells to tumor cell-killing effects were higher, the difference was significant both (P lt;0.01). 100:1 group and the 50:1 group, tumor cell-killing effect of the difference was not statistically significant (Pgt; 0.05); 100:1 group of tumor cell-killing effect of less than 20:1 group, the difference was statistically significant (P lt;0.05); 50:1 group of tumor cell-killing effect of less than 20:1 group, the difference was statistically significant (P lt;0.05). Conclusion: The human umbilical cord blood mononuclear cells in vitro cytokine-induced DC differentiation of cultured T cells can promote tumor cell-killing effect. Keywords: cord blood mononuclear cells, dendritic cells, immune function in tumor cells Enhancing Effect of Dendritic Ce