Umbilical cord blood CD34 cells in vitro expansion of HOXB4 gene expression changes when the.docVIP

 PAGE \* MERGEFORMAT 24 Umbilical cord blood CD34 cells in vitro expansion of HOXB4 gene expression changes when the Author: Yu-Hong Tang, Xiao-Ming Fei, Shen Yi, Miao Kou-Rong, Yu-Gui Cui, Cheng-Ya Abstract homeobox gene family member HOXB4 reflect the primitive hematopoietic stem / progenitor cells (PHSC / PHPC) self-renewal and proliferation. In this study, real-time quantitative RT-PCR method detected at the mRNA level of HOXB4 gene expression, in order to observe the in vitro expansion of umbilical cord blood CD34 cells, the level of self-renewal. The results showed that: With the extension of time in vitro, although the number of CD34 cells increased, but decreased expression of HOXB4; weeks later, HOXB4 is almost undetectable, and maturity level of HOXB4 expression in peripheral blood lymphocytes of the same; CD34 cells and bone marrow-derived mesenchymal stem cells (BM-MSC) co-cultured CD34 cells can slow the decline in HOXB4 expression. Conclusion: The umbilical cord blood CD34 cells in vitro amplification of the process of self-renewal capacity gradually decreased. CD34 and BM-MSC co-culture in vitro amplification help to reduce self-renewal capacity of CD34 cells in the loss. Keywords: cord blood CD34 cells in Expression of HOXB4 in Cord Blood Progenitor Cells Expanded in vitro Abstract HOXB4, a member of homeobox gene family, is closely related to the self-renewing and proliferative ability of primitive hematopoietic stem / progenitor cells (PHSC / PHPC). This study was aimed to investigate the self-renewing level of cord blood progenitor cells (CBPC) expanded in vitro. The HOXB4 expression at mRNA level was assayed by using real time RT-PCR. The results indicated that as culture prolonged, the total cells, CD34 cells greatly increased, however the HOXB4 expression gradually declined, even down to undetectable level similar to that of mature lymphocytes. Meanwhile, it was shown that CD34 cells co-cultured with bone