Wild-type PTEN in human ovarian cancer SKOV3 cell biological characteristics of the change.docVIP

 PAGE \* MERGEFORMAT 20 Wild-type PTEN in human ovarian cancer SKOV3 cell biological characteristics of the change Study: Ho Man Tang Xiaolong Wang Xuefeng Jiang Zhenyou Pangxue Yun Zhang Rongbo [Abstract] [Objective] The wild-type PTEN gene on the biological characteristics of ovarian cancer. [Method] The wild-type PTEN gene to the pAdxsi infected with adenovirus vector and SKOV3 cells. Fluorescence microscopic detection of virus on SKOV3 cells infection efficiency; with CCK-8 cell proliferation assay efficiency; RT-PCR and WB were used to detect the expression of PTEN mRNA and protein levels; immunochemical detection of PTEN and indirect fluorescence localization in cells and intracellular NEDD4- 1 and RAD51 molecules of expression. [results] stable transfection of PTEN after, 24 h within the PTEN distributed in the cytoplasm and nucleus, and 72 h, was mainly concentrated in the nucleus; a significant increase in the cytoplasm NEDD4-1 and thin nuclear RAD51, etc. molecule expression. [Conclusion] SKOV3 wild-type PTEN can affect cell proliferation and induced cells RAD51 and NEDD4-1 molecule, is conducive to cellular DNA repair and cell proliferation. [Keywords:] Transfection; NEDD4-1; PTEN gene Abstract: [Objective] To explore the effect of wild-type PTEN gene on biological characteristics of ovarian cancer cells. [Methods] The wild-type PTEN gene was cloned into pAdxsi adenovirus vector and infected into SKOV3 cells, the infected efficiency of adenovirus on SKOV3 cells was detected by fluorescence microscopy; the inhibition efficiency on cell proliferation was assayed by CCK-8; RT-PCR and WB was used to detect the level of PTEN mRNA and protein expression changes respectively; the location of PTEN NEDD4-1, RAD51 and other molecules in cell were showed by immunochemical and indirect fluorescence. [Results] The PTEN could be observed in the cytoplasm and nucleus after 24 h of stable transfection, while after 72 h they could be presented mainly in n