3种含笑属植物花粉生活力的测定.PDFVIP

( ) 安徽农业科学 ,Journal of Anhui Agri. Sci. 2007 ,35 1 :15 - 17 　　　　　　　　　　　　　　　　　　　　　　责任编辑　刘月娟　责任校对　孙能森　 3 种含笑属植物花粉生活力的测定 ( ) 杨琴军 , 黄英平 , 陈龙清　 华中农业大学园艺林学学院 ,湖北武汉 430070 摘要　以阔瓣含笑、平伐含笑、含笑为材料,对这3 个树种的花期进行跟踪观察 ,并且采用 TTC 染色法和固体培养基法对它们的新鲜花 粉和4 ℃低温下保存的花粉进行了生活力的测定。结果表明:培养基中蔗糖浓度对花粉萌发具有明显影响, 阔瓣含笑花粉的最佳萌发 浓度为 10 % ,而平伐含笑和含笑的最佳萌发浓度均为 15 % ;TTC 染色法测定的花粉生活力比萌发法高;4 ℃低温保存条件下,阔瓣含笑花 粉的可贮藏时间最短 ,约40 d 后花粉便失去了生活力 ,而含笑和平伐含笑的花粉贮藏40 d 后的萌发率分别为28. 77 % 、32. 01 % 。 关键词　含笑属;花期;花粉生活力 ( ) 中图分类号　Q944. 42 　　文献标识码　A 　　文章编号　0517 - 6611 2007 01 - 00015 - 03 Study on Pollen Viability of Three Species of Michelia ( ) YANG Qinjun et al 　 College of Horticulture and Forestry Sciences , Huazhong Agricultural University , Wuhan , Hubei 430070 Abstract 　The pollen viability of fresh pollen and preserved pollen from three species of Michelia were studied with TTC staining method and the solid culture medium method. The results showed that the concentration of sucrose effected on the pollen germinating rate significantly. The germinating rate of fresh pollen of Michelia platypetala reached the highest with 10 % sucrose , the highest germinating rate of fresh pollen of Michelia cavaleriei and Michelia figo with 15 % sucrose. The pollen viability tested by the TTC staining method was higer than that by gemination method. The study of 4 ℃cold storage showed that the pollen of Michelia platypetala lost viability nearly after 40 days and the pollen of Michelia cavaleriei and Michelia figo retained the viabili ty with 32. 01 % and 28. 77 % after 40 days. Key words 　 Michelia linn ; Florescence ; Pollen viability 　　木兰科含笑属植物约 50 种 ,主要分布于亚洲南部和东