软脂酸和花生四烯酸对人肝细胞瘤细胞株细胞葡萄糖摄取的影响_王平.pdfVIP

·140 · 2005 13 2　Chin J iabetes, April 2005, Vol 13, No 2 · · 王平　母义明　汪保安　潘长玉 【】　(HepG2), HepG2 2; , 。 【】　;; Ef fects of palmitic acid and arachidonic acid on the glucose uptake of HepG2 cell s　WA N G P ing , M U Yi-m ing, WA N G B ao-an , et al.D ep artm ent of E ndocrinology , Ch inese PL A General H osp ital, B ei ing 100853 Corresp ond ing author :M U Yi-m ing , E-mai l:m uy im ing @301 hosp 【Summary】　The study of effects of fatty acids on HepG2 cells showed that palmitic acid at high concentration decreased glucose uptake of HepG2 cells possibly through inhibiting the expression of Ins receptor and GluT2 proteins.Arachidonic acid stimulated glucose uptake through the similar mechanism with preventing the inhibitory effects of palmitic acid. 【Key words】　Palmitic acid;Arachidonic acid;Glucose uptake 4 　　(IR)2 (T2 M) 3.7×10 Bq/L, 15 min PBS 3 , 0.1 , 、。 mol/L NaOH 。BCA , 。 , (CPM )。 (HepG2), (PA) CPM , (AA) 。Western InsR GluT2 : , 。 HepG2 PA AA 24 h , 、 PBS 3 , S S [62.5 mmol/L T ris-HCI (pH 1.:PA、AA Sigma 。 6.8), 2%S S, 10%, 50 mmol/L T T, 0.2 mmol/L MEM 、GIBCO 。 PMSF 0.2 TIU/ml aprotinin, 0.7 μg/ml pepstatin 1 3 Hyclone 。2-deoxy-[ H]- -glucose μg/ml leupeptin] , 。 。HepG2 ATCC 。 100 μg S S, 2(GluT2)Chemicon , PV F , InsR GluT2 (InsR) Lab vision , ECL , HRP , ECL Santa Cruz 。 , Kodak 。 2.:HepG2 10% M EM 3.:Stata 。 , 37℃、5%CO 。, 、 2 HepG2 24