A Rapid MALDI-TOF MS Identification Database at Genospecies Level for Clinical and Environmental Aeromonas Strains 英文参考文献.docVIP
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A Rapid MALDI-TOF MS Identification Database at Genospecies Level for Clinical and Environmental Aeromonas Strains 英文参考文献
ARapidMALDI-TOFMSIdentificationDatabaseat
GenospeciesLevelforClinicalandEnvironmental
AeromonasStrains
CinziaBenagli1*,AntonellaDemarta1,AnnaPaolaCaminada1,DominikZiegler2,OrlandoPetrini1,
MauroTonolla1,3
1InstituteofMicrobiology,Bellinzona,Switzerland,2MabritecAG,Riehen,Switzerland,3MicrobialEcology,MicrobiologyUnit,PlantBiologyDepartmentUniversityof
Geneva,Gene`ve,Switzerland
Abstract
ThegenusAeromonashasundergoneanumberoftaxonomicandnomenclaturerevisionsoverthepast20years,andnew
(sub)species and biogroups are continuously described. Standard identification methods such as biochemical
characterization have deficiencies and do not allow clarification of the taxonomic position. This report describes the
development of a matrix-assisted laser desorption/ionisation–time of flight mass spectrometry (MALDI-TOF MS)
identificationdatabaseforarapididentificationofclinicalandenvironmentalAeromonasisolates.
Citation:BenagliC,DemartaA,CaminadaA,ZieglerD,PetriniO,etal.(2012)ARapidMALDI-TOFMSIdentificationDatabaseatGenospeciesLevelforClinicaland
EnvironmentalAeromonasStrains.PLoSONE7(10):e48441.doi:10.1371/journal.pone.0048441
Editor:MikaelSkurnik,UniversityofHelsinki,Finland
ReceivedMay10,2012;AcceptedSeptember25,2012;PublishedOctober31,2012
Copyright: ? 2012 Benagli etal. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits
unrestricteduse,distribution,andreproductioninanymedium,providedtheoriginalauthorandsourcearecredited.
Funding:Nocurrentexternalfundingsourcesforthisstudy.
CompetingInterests:Theauthorshavereadthejournal’spolicyandhavethefollowingconflicts:DominikZieglerisemployedbyMabritecAG.Thisdoesnot
altertheauthors’adherencetoallthePLOSONEpoliciesonsharingdataandmaterials.
*E-mail:cinzia.benagli@ti.ch
Introduction
[15,16,17,18,19,20]. One major problem in Aeromonas identifica-
tion relies on the fact that some species are phenotypically very
similar(e.g.A.caviaeandA.media,A.veroniiandA.sobria ).S
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