Acute Alterations of Somatodendritic Action Potential Dynamics in Hippocampal CA1 Pyramidal Cells after Kainate-Induced Status Epilepticus in Mice 英文参考文献.docVIP

下载本文档

3
0
约8.19万字
约 15页
2017-05-11 发布于上海
举报
版权申诉

Acute Alterations of Somatodendritic Action Potential Dynamics in Hippocampal CA1 Pyramidal Cells after Kainate-Induced Status Epilepticus in Mice 英文参考文献.doc

1、本文档共15页，可阅读全部内容。
2、原创力文档（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。
3、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击这里二次下载。
4、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。
5、该文档为VIP文档，如果想要下载，成为VIP会员后，下载免费。
6、成为VIP后，下载本文档将扣除1次下载权益。下载后，不支持退款、换文档。如有疑问请联系我们。
7、成为VIP后，您将拥有八大权益，权益包括：VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
8、VIP文档为合作方或网友上传，每下载1次，网站将根据用户上传文档的质量评分、类型等，对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档

Acute Alterations of Somatodendritic Action Potential Dynamics in Hippocampal CA1 Pyramidal Cells after Kainate-Induced Status Epilepticus in Mice 英文参考文献

AcuteAlterationsofSomatodendriticActionPotential DynamicsinHippocampalCA1PyramidalCellsafter Kainate-InducedStatusEpilepticusinMice DanielMinge,RobertBa¨hring* Institutfu¨rZellula¨re undIntegrativePhysiologie,Zentrumfu¨rExperimentelleMedizin,Universita¨tsklinikum Hamburg-Eppendorf,Hamburg,Germany Abstract Pathophysiological remodeling processes at an early stage of an acquired epilepsy are critical but not well understood. Therefore,weexaminedacutechangesinactionpotential(AP)dynamicsimmediatelyfollowingstatusepilepticus(SE)in mice.SEwasinducedbyintraperitoneal(i.p.)injectionofkainate,andbehavioralmanifestationofSEwasmonitoredfor3– 4h. After this time interval CA1 pyramidal cells were studied ex vivo with whole-cell current-clamp and Ca2+ imaging techniques in a hippocampal slice preparation. Following acute SE both resting potential and firing threshold were modestlydepolarized(2–5mV).Nochangeswereseenininputresistanceormembranetimeconstant,butAPlatencywas prolongedandAPupstrokevelocityreducedfollowingacuteSE.AllcellsshowedanincreaseinAPhalfwidthandregular (ratherthanburst)firing,andinafractionofcellsthenotch,typicallyprecedingspikeafterdepolarization(ADP),wasabsent followingacuteSE.Notably,thetypicalattenuationofbackpropagatingactionpotential(b-AP)-inducedCa2+signalsalong theapicaldendritewasstrengthenedfollowingacuteSE.TheeffectsofacuteSEontheretrogradespreadofexcitationwere mimickedbyapplyingtheKv4currentpotentiatingdrugNS5806.Ourdataunveilareducedsomatodendriticexcitabilityin hippocampal CA1 pyramidal cells immediately after acute SE with a possible involvement of both Na+ and K+ components. current Citation:MingeD,Ba¨hringR(2011)AcuteAlterationsofSomatodendriticActionPotentialDynamicsinHippocampalCA1PyramidalCellsafterKainate-Induced StatusEpilepticusinMice.PLoSONE6(10):e26664.doi:10.1371/journal.pone.0026664 Editor:GennadyCymbalyuk,GeorgiaStateUniversity,UnitedStatesofAmerica ReceivedJuly8,2011;AcceptedSeptember30,2011;PublishedOctober24,2011 Copyright: