AFM Probing the Mechanism of Synergistic Effects of the Green Tea Polyphenol (?)-Epigallocatechin-3-Gallate (EGCG) with Cefotaxime against Extended-Spectrum Beta-Lactamase (ESBL)-Producing Escherichia coli 英文参考文献.docVIP
- 1、本文档共6页,可阅读全部内容。
- 2、原创力文档(book118)网站文档一经付费(服务费),不意味着购买了该文档的版权,仅供个人/单位学习、研究之用,不得用于商业用途,未经授权,严禁复制、发行、汇编、翻译或者网络传播等,侵权必究。
- 3、本站所有内容均由合作方或网友上传,本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺!文档内容仅供研究参考,付费前请自行鉴别。如您付费,意味着您自己接受本站规则且自行承担风险,本站不退款、不进行额外附加服务;查看《如何避免下载的几个坑》。如果您已付费下载过本站文档,您可以点击 这里二次下载。
- 4、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等,请点击“版权申诉”(推荐),也可以打举报电话:400-050-0827(电话支持时间:9:00-18:30)。
- 5、该文档为VIP文档,如果想要下载,成为VIP会员后,下载免费。
- 6、成为VIP后,下载本文档将扣除1次下载权益。下载后,不支持退款、换文档。如有疑问请联系我们。
- 7、成为VIP后,您将拥有八大权益,权益包括:VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
- 8、VIP文档为合作方或网友上传,每下载1次, 网站将根据用户上传文档的质量评分、类型等,对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档
查看更多
AFM Probing the Mechanism of Synergistic Effects of the Green Tea Polyphenol (?)-Epigallocatechin-3-Gallate (EGCG) with Cefotaxime against Extended-Spectrum Beta-Lactamase (ESBL)-Producing Escherichia coli 英文参考文献
AFMProbingtheMechanismofSynergisticEffectsofthe
GreenTeaPolyphenol(2)-Epigallocatechin-3-Gallate
(EGCG)withCefotaximeagainstExtended-Spectrum
Beta-Lactamase(ESBL)-ProducingEscherichiacoli
YidanCui1,3,SoHyunKim1,HyunseokKim1,JinkiYeom2,KisungKo4,WoojunPark2,SungsuPark1,3*
1DepartmentofChemistryandNanoSciences,EwhaWomansUniversity,Seoul,Korea,2DivisionofEnvironmentalScienceandEcologicalEngineering,KoreaUniversity,
Seoul,Korea,3MechanobiologyInstitute,NationalUniversityofSingapore,Singapore,Singapore,4DepartmentofMedicine,CollegeofMedicine,Chung-AngUniversity,
Seoul,Korea
Abstract
Background: Extended-spectrum b-lactamase (ESBL)-producing Enterobacteriaceae poses serious challenges to clinicians
becauseofitsresistancetomanyclassesofantibiotics.
Methods and Findings: The mechanism of synergistic activity of a combination of (2)-epigallocatechin-3-gallate (EGCG)
andb-lactamantibioticscefotaximewasstudiedonExtended-spectrumb-lactamaseproducingEscherichiacoli(ESBL-EC),by
visualizingthemorphologicalalterationonthecellwallinducedbythecombinationusingatomicforcemicroscopy(AFM).
Cellsatsub-MICs(sub-minimuminhibitoryconcentrations)ofcefotaximewereinitiallyfilamentatedbutrecoveredtothe
normalshapelater,whereascellsatsub-MICsofEGCGexperiencedtemporaldisturbanceonthecellwallsuchasleakage
andreleaseofcellulardebrisandgrooveformation,butlaterrecoveredtothenormalshape.Incontrast,thecombinationof
cefotaximeandEGCGattheirrespectivesub-MICsinducedpermanentcellulardamagesaswellascontinuouselongationin
cells and eventually killed them. Flow cytometry showed that intracellular oxidative stress levels in the cell treated with
acombinationofEGCGandcefotaximeatsub-MICswerehigherthanthoseinthecellstreatedwitheithercefotaximeor
EGCGatsub-MICs.
Conclusions:TheseresultssuggestthatthesynergisticeffectofEGCGbetweenEGCGandcefotaximeagainstESBL-ECis
related to cooperative activity of exogenous and endogenous reactive oxygen species (ROS) generated by EGCG and
cefotaxime,respectively.
Citation:CuiY,Ki
您可能关注的文档
- Adjuvanted Influenza Vaccine Administered Intradermally Elicits Robust Long-Term Immune Responses that Confer Protection from Lethal Challenge 英文参考文献.doc
- Adjusting HIV Prevalence for Survey Non-Response Using Mortality Rates An Application of the Method Using Surveillance Data from Rural South Africa 英文参考文献.doc
- Adjusting Cholera Models to Recent Experimental Data 英文参考文献.doc
- ADMA in Vascular Disease More than a Marker 英文参考文献.doc
- Adiposity Predicts Cognitive Decline in Older Persons with Diabetes A 2-Year Follow-Up 英文参考文献.doc
- Administration of Triclabendazole Is Safe and Effective in Controlling Fascioliasis in an Endemic Community of the Bolivian Altiplano 英文参考文献.doc
- Administration of Simvastatin after Kainic Acid-Induced Status Epilepticus Restrains Chronic Temporal Lobe Epilepsy 英文参考文献.doc
- Adjacent Nucleotide Dependence in ncRNA and Order-1 SCFG for ncRNA Identification 英文参考文献.doc
- Adjunctive TNF Inhibition with Standard Treatment Enhances Bacterial Clearance in a Murine Model of Necrotic TB Granulomas 英文参考文献.doc
- Adjusting Phenotypes by Noise Control 英文参考文献.doc
文档评论(0)