Agglutinating Activity and Structural Characterization of Scalarin, the Major Egg Protein of the Snail Pomacea scalaris (d’Orbigny, 1832) 英文参考文献.docVIP
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Agglutinating Activity and Structural Characterization of Scalarin, the Major Egg Protein of the Snail Pomacea scalaris (d’Orbigny, 1832) 英文参考文献
AgglutinatingActivityandStructuralCharacterizationof
Scalarin,theMajorEggProteinoftheSnailPomacea
scalaris(d’Orbigny,1832)
SantiagoItuarte1,MarcosSebastia′nDreon1,2*,MarceloCeolin3,HoracioHeras1,4
1Instituto de Investigaciones Bioqu?′micas de La Plata (INIBIOLP), CONICET CCT La Plata - Universidad Nacional de La Plata (UNLP), La Plata, Argentina, 2Ca′tedra de
Bioqu?′mica y Biolog?′a Molecular, Fac. de Cs. Me′dicas - Universidad Nacional de La Plata (UNLP), La Plata, Argentina, 3Instituto de Investigaciones F?′sicas Teo′ricas y
Aplicadas(INIFTA),CONICETCCTLaPlata-UniversidadNacionaldeLaPlata(UNLP),LaPlata,Argentina,4Ca′tedra deQu?′micaBiolo′gica,Fac.deCs.NaturalesyMuseo-
UniversidadNacionaldeLaPlata(UNLP),LaPlata,Argentina
Abstract
Apple snail perivitellins are emerging as ecologically important reproductive proteins. To elucidate if the protective
functionsoftheeggproteinsofPomaceacanaliculata(Caenogastropoda,Ampullariidae),involvedinembryodefenses,are
presentinotherPomaceaspecieswestudiedscalarin(PsSC),themajorperivitellinofPomaceascalaris.UsingsmallangleX-
rayscattering,fluorescenceandabsorptionspectroscopyandbiochemicalmethods,weanalyzedPsSCstructuralstability,
agglutinatingactivity,sugarspecificityandproteaseresistance.PsSCaggluttinatedrabbit,and,toalesserextent,humanB
and A erythrocytes independently of divalent metals Ca2+ and Mg were strongly inhibited by galactosamine and
glucosamine. The protein was structurally stable between pH2.0 to 10.0, though agglutination occurred only between
pH4.0to8.0(maximumactivityatpH7.0).Theagglutinatingactivitywasconservedupto60uCandcompletelylostabove
80uC, in agreement with the structural thermal stability of the protein (up to 60uC). PsSC was able to withstand invitro
gastrointestinaldigestion,andshowednotrypsininhibitionactivity.Thepresenceoflectinactivityhasbeenreportedin
eggsofotherPomaceasnails,butherewelinkforthefirsttime,thisactivitytoanapplesnailmultifunctionalperivitellin.
Thisnovelroleforasnaileggstorage
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