Artificial Skin – Culturing of Different Skin Cell Lines for Generating an Artificial Skin Substitute on Cross-Weaved Spider Silk Fibres 英文参考文献.docVIP
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Artificial Skin – Culturing of Different Skin Cell Lines for Generating an Artificial Skin Substitute on Cross-Weaved Spider Silk Fibres 英文参考文献
ArtificialSkin–CulturingofDifferentSkinCellLinesfor
GeneratinganArtificialSkinSubstituteonCross-Weaved
SpiderSilkFibres
HannaWendt1*.,AnjaHillmer1.,KerstinReimers1,JoernW.Kuhbier1,FranziskaScha¨fer-Nolte1,
ChristinaAllmeling1,CorneliaKasper2,PeterM.Vogt1
1Department of Plastic, Hand, and Reconstructive Surgery, Medical School Hannover, Hannover, Germany, 2Institute of Technical Chemistry, Leibniz University of
Hannover,Hannover,Germany
Abstract
Background:InthefieldofPlasticReconstructiveSurgerythedevelopmentofnewinnovativematricesforskinrepairisin
urgent need. The ideal biomaterial should promote attachment, proliferation and growth of cells. Additionally, itshould
degrade in an appropriate time period without releasing harmful substances, but not exert a pathological immune
response.SpiderdraglinesilkfromNephilasppmeetsthesedemandstoalargeextent.
Methodology/PrincipalFindings:Nativespiderdraglinesilk,harvesteddirectlyoutofNephilasppspiders,waswovenon
steel frames. Constructs were sterilized and seeded with fibroblasts. After two weeks of cultivating single fibroblasts,
keratinocyteswereaddedtogenerateabilayeredskinmodel,consistingofdermisandepidermisequivalents.Forthenext
threeweeks,constructsinco-culturewereliftedonanoriginallydesignedsetupforair/liquidinterfacecultivation.Afterthe
culturing period, constructs were embedded in paraffin with an especially developed program for spidersilk to avoid
supercontraction.Paraffincross-sectionswerestainedinHaematoxylinEosin(HE)formicroscopicanalyses.
Conclusion/Significance:Nativespiderdraglinesilkwovenonsteelframesprovidesasuitablematrixfor3dimensionalskin
cell culturing. Bothfibroblasts and keratinocytes cell lines adhere to the spider silk fibres andproliferate. Guided by the
spider silk fibres, they sprout into the meshes and reach confluence in at most one week. A well-balanced, bilayered
cocultivationintwocontinuouslyseparatedstratacanbeachievedbyserumreduction,changingthemediumconditions
andthecultivationperiodatt
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