检测丙型肝炎病毒F抗体的一种间接ELISA的初步评价.pdfVIP

.771. 文.编号;1673-8ω0(2010) 10-0771 -04 中阁分类号:民钊6.61 文献栋t只码:A 检测丙型肝炎病毒 F 抗体的一种间接 ELISA 的初步评价 武丈娥1 ， 邵圣文2 ， 巴剑波1 ， 戚中回3 (1.海军医学研究所王室，上海 2ω433;2. 湖州师范学院院学院微生物学教研室，浙江湖州 3130∞; 3. 第二军医大学微生物学教研室，上海 2ω433) 摘襄:目的 评价以两型肝炎病毒(HCV) F 蛋白为包被抗原的间接酶联免疫吸附试验(ELISA) 的准确性和 可靠性。方法 以基因工程重组 HCV F 抗原包被酶标微扎板，检测 HCV 感染者血清抗J 滴度，建在阳性判断 值，计算 HCV 感染者血清抗嗣F 阳性率。与某市售 HCV ELISA 试剂同时检测 HCV 感染者和非感染者抗J 阳性 率，计算该 ELISA 的敏感性和特异性等技术指栋。结果 该间接 ELISA 槛测的敏感性为 66. 79岛，特异性为 96.7% ，正确指数为63.3% ，一致率为 81. 79岛 ， Kappa 值为0.63 ，变异系敬(CV) 为6.23% 。结论该间接ELISA 初步评价指标符合准确性和可靠性摆求，具有潜在补充枪测 HCV 感染的扩腾应用价值。 关键词:两型肝炎病毒;F 抗体;间接酶联兔班吸附试验;评价 Primary evalua份。n of 栩如dir酬 ELISA for dete耐ng Hepa硝îs C virus anti-F antibody WU Wenbinl , SHAO Slumgwen2 , BA Jianbo l , QI Zho略1肌3 (l. The Third Department , Navy Medical lnstit脚， Shanghai 2∞433 ， China; 2. Department of Microbiology , Huzhou Teachers College Sdωol of Medicine , Zhejlωng Huz加u 3日创泊， Chi附; 3. Department of Microb切，logy ， Second Míli，ωry Medical Univers时， Shar问ghai 2α)433 ， China) Abstract: Objective To evaluate the accuracy and reliabílity of an indirect enzyme-linked immunosorbentωsay (览USA) for detecting antibody 10 Hepαtitis C virus (HCV) F protein. Methods 四e recombinant HCV F prolein w踊 coated in a 96幽well microplate 8s a capture antigen for detecting the anti-F in sera of patients infected with HCV. Mter the establishment of the cut-off value , the positive rate of anti-F in patients infected with HCV was calculated. Compared with other HCV ELISA kits , the sensitivity. specificity and other parameters of the indirect EUSA were calculated. Results 叽le sensítivity and specificity of the indirect ELISA were 66.7% and 96. 7%. The accuracy index was 63.3% , the concordance wω8， 1. 79岛， and the Kappa value wω0.63. The coefficient of variation