Quantitative Photo Activated Localization Microscopy Unraveling the Effects of Photoblinking 英文参考文献.docVIP

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Quantitative Photo Activated Localization Microscopy Unraveling the Effects of Photoblinking 英文参考文献.doc

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Quantitative Photo Activated Localization Microscopy Unraveling the Effects of Photoblinking 英文参考文献

QuantitativePhotoActivatedLocalizationMicroscopy: UnravelingtheEffectsofPhotoblinking PaoloAnnibale1.,StefanoVanni2.,MarcoScarselli1.,UrsulaRothlisberger2,AleksandraRadenovic1* 1Laboratory of Nanoscale Biology, Institute of Bioengineering, Ecole Polytechnique Fe′de′rale de Lausanne, Lausanne, Switzerland, 2Laboratory of Computational ChemistryandBiochemistry,InstituteofChemicalSciencesandEngineering,EcolePolytechniqueFe′de′rale deLausanne,Lausanne,Switzerland Abstract In this work we discuss how to use photophysical information for improved quantitative measurements using Photo Activated Localization Microscopy (PALM) imaging. We introduce a method that reliably estimates the number of photoblinkingmoleculespresentinabiologicalsampleandgivesarobustwaytoquantifyproteinsatthesingle-celllevel fromPALMimages.Weapplythismethodtodeterminetheamountofb2adrenergicreceptor,aprototypical GProtein CoupledReceptor,expressedontheplasmamembraneofHeLacells. Citation:AnnibaleP,VanniS,ScarselliM,RothlisbergerU,RadenovicA(2011)QuantitativePhotoActivatedLocalizationMicroscopy:UnravelingtheEffectsof Photoblinking.PLoSONE6(7):e22678.doi:10.1371/journal.pone.0022678 Editor:RainerHeintzmann,Friedrich-Schiller-UniversityJena,Germany ReceivedMarch10,2011;AcceptedJune30,2011;PublishedJuly26,2011 Copyright: ? 2011 Annibale et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricteduse,distribution,andreproductioninanymedium,providedtheoriginalauthorandsourcearecredited. Funding:ThisworkwasfinanciallysupportedbyFNSgrantsno.200021-125319,no20021-132206,andCRSII3_132396(http://www.snf.ch/E/Pages/default.aspx). Thefundershadnoroleinstudydesign,datacollectionandanalysis,decisiontopublish,orpreparationofthemanuscript. CompetingInterests:Theauthorshavedeclaredthatnocompetinginterestsexist. *E-mail:aleksandra.radenovic@epfl.ch .Theseauthorscontributedequallytothiswork. Introduction original number of molecu