Species Discrimination, Population Structure and Linkage Disequilibrium in Eucalyptus camaldulensis and Eucalyptus tereticornis Using SSR Markers 英文参考文献.docVIP
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Species Discrimination, Population Structure and Linkage Disequilibrium in Eucalyptus camaldulensis and Eucalyptus tereticornis Using SSR Markers 英文参考文献
SpeciesDiscrimination,PopulationStructureand
LinkageDisequilibriuminEucalyptuscamaldulensisand
EucalyptustereticornisUsingSSRMarkers
ShanmugapriyaArumugasundaram1,ModhumitaGhosh1,SivakumarVeerasamy2 ,Yasodha
Ramasamy1*
1Division ofPlant Biotechnology, Institute ofForestGenetics andTreeBreeding,Coimbatore, Tamilnadu,India, 2DivisionofGenetics andTreeBreeding, Institute of
ForestGeneticsandTreeBreeding,Coimbatore,Tamilnadu,India
Abstract
EucalyptuscamaldulensisandE.tereticornisarecloselyrelatedspeciescommonlycultivatedforpulpwoodinmanytropical
countriesincludingIndia.Understandingthegeneticstructureandlinkagedisequilibrium(LD)existinginthesespeciesis
essentialfortheimprovementofindustriallyimportanttraits.Ourgoalwastoevaluatetheuseofsimplesequencerepeat
(SSR)lociforspeciesdiscrimination,populationstructureandLDanalysisinthesespecies.Investigationswerecarriedout
with the most common alleles in 93 accessions belonging to these two species using 62 SSR markers through cross
amplification.Thepolymorphicinformationcontent(PIC)rangedfrom0.44to0.93and0.36to0.93inE.camaldulensisand
E.tereticornisrespectively.Acleardelineationbetweenthetwospecieswasevidentbasedontheanalysisofpopulation
structureandspecies-specificalleles.SignificantgenotypicLDwasfoundinE.camaldulensis,whereinoutof135significant
pairs,17pairsshowedr2$0.1.Similarly,inE.tereticornis,outof136significantpairs,18pairsshowedr2$0.1.Theextentof
LD decayed rapidly showing the significance of association analyses in eucalypts with higher resolution markers. The
availabilityofwholegenomesequenceforE.grandisandthesyntenyandco-linearityinthegenomeofeucalypts,willallow
genome-widegenotypingusingmicrosatellitesorsinglenucleotidepolymorphims.
Citation:ArumugasundaramS,GhoshM,VeerasamyS,RamasamyY(2011)SpeciesDiscrimination,PopulationStructureandLinkageDisequilibriuminEucalyptus
camaldulensisandEucalyptustereticornisUsingSSRMarkers.PLoSONE6(12):e28252.doi:10.1371/journal.pone.0028252
Editor:Pa¨r K.Ingvarsson,UniversityofUme
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