原创力文档Analysis and application of Bacillus subtilis sortases to anchor recombinant proteins on the cell wall.docVIP
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Analysis and application of Bacillus subtilis sortases to anchor recombinant proteins on the cell wall
Nguyenetal.AMBExpress2011,1:22
/content/1/1/22
ORIGINAL
OpenAccess
AnalysisandapplicationofBacillussubtilis
sortasestoanchorrecombinantproteinson
thecellwall
HoangDucNguyen1,2,3*,TrangThiPhuongPhan1,2,3 andWolfgangSchumann1
Abstract
Bacillussubtiliscodesfortwoputativesortases,YhcSandYwpE,andtwosurfaceproteins,YhcRandYfkN,harboring
sortingmotifssupposedtoberecognizedbytheputativesortase(s).However,thereisnoexperimentalevidence
toshowadirectlinkbetweenthesesortasesandsortingsequences.Tostudytheroleofthesetwoputative
sortasesondisplayingYhcRandYfkNonthecellwall,expressionofyhcSandywpEwasanal
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