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Analyzing and minimizing PCR amplification bias in Illumina sequencing libraries
Airdetal.GenomeBiology2011,12:R18
/2011/12/2/R18
METHOD
OpenAccess
AnalyzingandminimizingPCRamplificationbias
inIlluminasequencinglibraries
DanielAird1,MichaelGRoss1,Wei-ShengChen2,MaxwellDanielsson2,TimothyFennell3,CarstenRuss1,
DavidBJaffe1,ChadNusbaum1,AndreasGnirke1*
Abstract
Despitetheever-increasingoutputofIlluminasequencingdata,lociwithextremebasecompositionsareoften
under-representedorabsent.Toevaluatesourcesofbase-compositionbias,wetracedgenomicsequencesranging
from6%to90%GCthroughtheprocessbyquantitativePCR.WeidentifiedPCRduringlibrarypreparationasa
principalsourceofbiasandoptimize
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