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抑癌蛋白UBIAD1的细胞亚定位以及它与H-Ras、ApoE的相互作用的研究.pdf 70页

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抑癌蛋白UBIAD1的细胞亚定位以及它与H-Ras、ApoE的相互作用的研究
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华 中 科 技 大 学 硕 士 学 位 论 文 Abstract TERE1 (Transitional epithelial response gene 1) was found and cloned the first time by Terence W McGarvey et al in 2001. Due to the fact that TERE1 containing the UbiA prenyltransferase domain containing 1 , TERE1 is also called UBIAD1. UBIAD1 gene is ubiquitously expressed in normal human tissues. The open reading frame of UBIAD1 gene encode a 8-10 times α-helical transmembrane protein of 36.8 KD and 338 amino acids. UBIAD1 gene is related with many human diseases, such as transitional cell carcinoma of the bladder, Schnyder crystalline corneal dystrophy and Parkinson’s disease. However, until now people haven ’t known the role of UBIAD1 gene in these diseases yet, which may be due to the differences understanding of the UBIAD1 protein subcellular localization. In our study, we find the UBIAD1 protein located on the ER and Golgi apparatus, but not on the membrane. And we find the protein UBIAD1 is enriched in Golgi isolated from L02 cells. After the subcellular localization of UBIAD1 is identified, we began to study its function. We observed the protein UBIAD1, ApoE and H-Ras are colocalized on the Golgi apparatus. And we observed the fluorescence resonance energy transformation phenomenon among the three proteins. In Co-Immunoprecipitation experiments, we observed the UBIAD1 protein can interact respectly with ApoE and H-Ras, respectly. We also observed that ApoE can induce apoptosis of bladder carcinoma T24 cells, which indicates the protein complex, ApoE-UBIAD1-H-Ras, can induce apoptosis of tumor cells. Key words: UBIAD1, H-Ras, ApoE, subcellular localization, protein-protein interaction, bladder carcinoma

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