养分胁迫对威优916生育后期叶鞘衰老影响的蛋白质组分析.pdfVIP

I C , I M刷CULTURAE 华北农学报. 2011 , 26(3) :106-112 舷IU-11翩翩 养分胁迫对威优916 生青后期叶翰衰老影晌 的蛋白质组分析 谢金水1 ， 2 ，邵彩虹2 ，唐秀英1 ，刘光荣2 ，石庆华1 3302∞} 33∞45;2. 江西省农业科学院土攘肥料与资源环珑研究所，江西南昌 (1.江西农业大学农学院，江西南昌 摘要:从蛋白质组学角度，对养分胁迫下杂交水稻威优916 早衰过程中叶销的蛋白质差异表达进行分析，以揭示 叶鞠衰老的分子机理。水稻生育后期设清水及常规营养液培养两种处理，分4 个时期提取叶鞠蛋白质，经双向电泳 分离得到电泳图谱8 张，应用 Imagemasler 2D Elite 5.0 软件对其进行蛋白质差异表达分析，共得到 27 个发生差异表 达蛋白质点，其中 19 个经质谱分析功能得到鉴定。综合分析相关蛋白质的功能及其表达量变化，结果显示，养分胁 迫诱导叶销中大量的抗性及胁迫应答蛋白质发生差异表达，叶销的光合性能降低，呼吸消耗增强，干物质积累减少， 导致叶辅的早衰发生。 关键词:水稻;养分胁迫:叶销;衰老;蛋白质组学 中图分类号:5312; 551 1. 01 文献标识码:A 文章编号:1000 -7091(2011)03 -0106 -07 Proteomics Analysis of Rice Sheath Senescence under Nutrient Stress Condition during Late Development Stage 1 2 1 2 1 XIE Jin-shui ,2 , SHAO Cai-hong , TANG Xiu-ying , LIU Guang-rong , SHI Qing-hua (1. College of Agronomy , Jiangxi Agricultural University , Nanchang 330045 ,China; 2. Soil and Fertilizer and Resource and Environment InstÏtute ,Jiangxi Academy of Agriculture Sciences ,Nanchang 330200 ,China) Abstract: In order to reveal the molecule mechanism of rice sheath premature senescence , differential expres- sion proteins of hyhrid Weiyou916 sheath were analyzed hy suing approach of plant proteomics. Rice were cultured with pure water and normal nutrient solution at late development stage. The sheath proteins were extracted from four different times respectively and investigated by 2-DE. There 8 maps were obtained and analyzed hy Imagemaster 2D Elite 5. O. Software quantification showed that 27 proteins expression ahundance changed in response to nutrient