DNA markers use common problems(国外英文资料).docVIP

DNA markers use common problems(国外英文资料) 1, the DNA tape is invisible or light, probable cause and solution A) DNA Marker is not enough Follow the instructions and adjust the sample according to your own experiment B) DNA electrophoresis runs out of the gel In electrophoresis, you can stop the electrophoresis by running the bromophenol blue to two thirds of the gel. Smaller pieces of DNA may require a shorter range of electrophoresis. C) DNA strips are covered by tracer dye Use different electrophoresis, loading dye, to avoid interference with the tracer dye, or to lower the amount of loading dye. D) the nucleic acid stain is not sufficient in the gel or in the back dye. For example, the gel thats stored overnight will break down a lot, and the next day, the DNA band will be weakened significantly. Therefore, use EB as the gel of the nucleic acid dye, which is the best day to make and use. A few remaining gels, if you want to use them the next day, need to be added to the gel surface with the appropriate buffer, and preferably with a sealing cap. A band is separated from the stripe A) DNA is partially degraded. B) the voltage in the electrophoresis is too low to allow the DNA stripe to spread. C) the DNA is too large, the strip thickens, and the strips are not easily separated. D) properly reducing the added volume can improve the electrophoresis belt significantly. E) use an unappropriate concentration of AGAR gel. The gel was reprepared by the effective separation range of the concentrations of agar-agar gel. F) gelatin is of poor quality and is used to make gel with high quality agar-agar. The Marker front band is easily weakened or lost in the electrophoresis. Using EB as a nucleic acid dye, the Epstein is weak in the binding force of the DNA, and EB is in the opposite direction of the DNAs electrophoresis. When the DNA stripe runs to the front of the EB in the electrophoresis, there is a large part of the EB that goes away from the DNA and the strands of DNA wi