p38 MAPK在嗜酸性粒细胞活化支气管上皮细胞释放MCP_0.docVIP

p38 MAPK在嗜酸性粒细胞活化支气管上皮细胞释放MCP 作者：王成彬，童红莉，田亚平,汪德清，黄振国，叶伟基，李洛谊，林伟基 论文联盟www.LWLM.com编辑。【关键词】 上皮细胞 Regulatory role of p38 MAPK for MCP1 release from activated bronchial epithelial cells by eosinophils 　　【Abstract】 AIM: To investigate the release of monocyte chemoattractant protEin （MCP）1 from the coculture of human bronchial epithelial cells and eosinophils and the related mechanisms. METHODS: MCP1 in culture supernatant was determined by Cytometric Bead Array (CBA) Kit in flow cytometry to compare MCP1 release in bronchial epithelial cells and eosinophils cultured alone or together, and investigate the inhibitive effect of SB 203580, a selective inhibitor of p38 MAPK, on MCP1 release. The reverse transcriptasepolymerase chain reaction (RTPCR) was used to analyze the gene expression of MCP1 in bronchial epithelial cells during coculture with eosinophils and the effect of SB 203580. RESULTS: The interaction of eosinophils and bronchial epithelial cells was found to upregulate the gene expression of MCP1 in epithelial cells. MCP1 in coculture supernatant of bronchial epithelial cells and eosinophils was elevated significantly ［(1266±127) ng/L vs (134±25) ng/L,P0.001］. Fixed eosinophils, which lost the ability to release MCP1, could also elevate epithelial cells to release MCP1 ［(773±48) ng/L vs (107±15) ng/L, P0.001］ in coculture. SB 203580 could effectively inhibit MCP1 gene expression of bronchial epithelial cells during coculture with eosinophils, and decreased the release of MCP1 in the coculture of epithelial cells and eosinophils or fixed eosinophils ［(1335±115) ng/L vs (481±42) ng/L, (868±89) ng/L vs (239±26) ng/L, P0.001］. CONCLUSION: Eosinophils can activate bronchial epithelial cells to express MCP1 in coculture by p38 MAPK pathway so as to regulate the airway inflammatory reaction. 　　【Keywords】 bronchi; epithelial cells; eosinophils; monocyte chemoattractant protEIn1; p38 MAPK 　　【摘要】　目的： 探讨支气管上皮细胞与嗜酸性粒细胞联合培养过程中炎性介质的释放及其机制. 方法 ： 应用 流式细胞微珠实验（CBA）方法定量比较嗜酸性